| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 685,138 | C→G | 16.4% | V180V (GTG→GTC) | gltL ← | glutamate and aspartate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 685,138 | 0 | C | G | 16.4% | 135.7 / 17.8 | 61 | V180V (GTG→GTC) | gltL | glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (19/32); new base G (5/5); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATC > W3110S.gb/685070‑685207 | gCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTccac < 1:2962707/71‑1 (MQ=255)gCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca < 1:2066458/70‑1 (MQ=255)gCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca < 1:2943819/70‑1 (MQ=255)gCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca < 1:134637/70‑1 (MQ=255) gCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg > 1:819103/1‑71 (MQ=255) cAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg > 1:2279155/1‑70 (MQ=255) cAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg > 1:2929439/1‑70 (MQ=255) aaGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTAc < 1:1541697/71‑1 (MQ=255) ccATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCg > 1:1963109/1‑71 (MQ=255) ccATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCg < 1:1457689/71‑1 (MQ=255) aTTTCGTGGGTCACCACCATCATGGTAATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGtt < 1:976217/71‑1 (MQ=255) tttCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACtt < 1:122123/66‑1 (MQ=255) tCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat > 1:3188420/1‑71 (MQ=255) cGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat < 1:3387689/70‑1 (MQ=255) gTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatca < 1:1328897/71‑1 (MQ=255) gggTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatcatc > 1:1178370/1‑71 (MQ=255) caccacCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCg > 1:2653190/1‑71 (MQ=255) accaccATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTc < 1:1565715/47‑1 (MQ=255) accaccATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTc < 1:1282903/47‑1 (MQ=255) caccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCa > 1:631294/1‑47 (MQ=255) accaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATc < 1:2817364/71‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATc < 1:2636018/38‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGt < 1:3299282/47‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGt < 1:3702618/47‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGt < 1:228249/47‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGt < 1:1055531/47‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGt < 1:1011919/47‑1 (MQ=255) ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGt < 1:1789613/47‑1 (MQ=255) atcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTc < 1:907312/41‑1 (MQ=255) atcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTc < 1:2522474/41‑1 (MQ=255) atcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAg > 1:2054932/1‑71 (MQ=255) tcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTc < 1:1690963/49‑1 (MQ=255) catGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAgcg > 1:3316477/1‑71 (MQ=255) atGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGt > 1:3585996/1‑49 (MQ=255) atGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCg < 1:1146748/61‑1 (MQ=255) atGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAgcg < 1:1963719/70‑1 (MQ=255) atGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAgcg < 1:951119/70‑1 (MQ=255) tcgTCATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACTTCGTTGATCATCTCCGGATcc < 1:2346172/63‑1 (MQ=255) tcgTCATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACTTCGTTGATCATCTCCGGATcc < 1:644860/63‑1 (MQ=255) tGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatca < 1:1379586/54‑1 (MQ=255) tGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGcc < 1:450179/71‑1 (MQ=255) tGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGcc < 1:740345/71‑1 (MQ=255) tCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGa < 1:3441617/48‑1 (MQ=255) tCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATAATCTCCGGATCCAGCGCCGAt > 1:336401/1‑71 (MQ=255) cATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACt > 1:1305430/1‑40 (MQ=37) cATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACt > 1:2208273/1‑40 (MQ=37) cATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACt > 1:1380837/1‑40 (MQ=37) cATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACt > 1:892836/1‑40 (MQ=37) cATTCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACt > 1:2608183/1‑40 (MQ=37) cATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAg > 1:2157139/1‑63 (MQ=255) aTTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTTCTTCGTTGATCATCTCCGGATCCAGCGCCGATGt > 1:3325357/1‑71 (MQ=255) aTTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat > 1:1433073/1‑47 (MQ=255) aTTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat > 1:87684/1‑47 (MQ=255) aTTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGt < 1:110122/71‑1 (MQ=255) aTTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGt < 1:1932545/71‑1 (MQ=255) ttCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACTTCGTTGATCATCTCCGGATCCa < 1:3490244/60‑1 (MQ=255) ttCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACTTCGTTGATCATCTCCGGATCCa < 1:1573829/60‑1 (MQ=255) ttCCTTCGTTCGCCAGTTCGACCATCACGTCCAGCACTTCGTTGATCATCTCCGGATCCa < 1:2242163/60‑1 (MQ=255) tCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat > 1:3142916/1‑45 (MQ=255) ccTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCgg > 1:861692/1‑71 (MQ=255) ccTTCGTTCGCCAGTTCCACCATCACGTCAAGTAc > 1:860552/1‑35 (MQ=255) cTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCgg < 1:214484/70‑1 (MQ=255) ttcgttcgCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc < 1:17617/43‑1 (MQ=255) ttcgttcgCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGt < 1:1833715/70‑1 (MQ=255) gttcgCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGt < 1:1312514/71‑1 (MQ=255) ccaccaTCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGa > 1:300410/1‑49 (MQ=255) ccaccaTCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATc > 1:506479/1‑71 (MQ=255) ccaccaTCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATc > 1:2890528/1‑71 (MQ=255) | GCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATC > W3110S.gb/685070‑685207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |