| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 686,570 | T→G | 19.1% | S174S (TCA→TCC) | gltJ ← | glutamate and aspartate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 686,570 | 0 | T | G | 19.1% | 62.9 / 19.6 | 42 | S174S (TCA→TCC) | gltJ | glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/20); new base G (3/5); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAG > W3110S.gb/686501‑686638 | cTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTGACCAGGTTCATCATCTCTg < 1:3460614/71‑1 (MQ=255)cTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTg < 1:224041/71‑1 (MQ=255) cgccgcCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAgg > 1:2892505/1‑71 (MQ=255) ccgccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTc < 1:2617117/71‑1 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATctgcgg > 1:250751/1‑71 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcg > 1:2777054/1‑70 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCCGAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATcggcgg < 1:416427/71‑1 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCCGAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATcggcgg < 1:1818854/71‑1 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCCGAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATcggcgg < 1:725692/71‑1 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCCGAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATcggcgg < 1:3585377/71‑1 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCCGAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATcggcgg < 1:1234117/71‑1 (MQ=255) cGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATaa > 1:666869/1‑69 (MQ=255) aTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGt > 1:2037005/1‑50 (MQ=255) tAGTCGAGGCGATGGGGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGa > 1:2988601/1‑71 (MQ=255) aGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGa > 1:202576/1‑70 (MQ=255) cGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcgg > 1:594507/1‑51 (MQ=255) gAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAg > 1:1214918/1‑70 (MQ=255) ggCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCAt < 1:2448159/71‑1 (MQ=255) gATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAg < 1:2538668/71‑1 (MQ=255) ggCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAgg > 1:1742245/1‑69 (MQ=255) gCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGgcag < 1:1966652/71‑1 (MQ=255) gAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGcagcag > 1:2502914/1‑71 (MQ=255) gAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGcagcag > 1:1586370/1‑71 (MQ=255) gAGTTTTTCACCAGGTTCATCATCTCGGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGcagcag > 1:354208/1‑71 (MQ=255) aGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGt < 1:68467/71‑1 (MQ=255) aGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGt < 1:1162674/71‑1 (MQ=255) tttCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACg < 1:471505/70‑1 (MQ=255) tttCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACg < 1:904646/70‑1 (MQ=255) tttCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACg < 1:1336008/70‑1 (MQ=255) aGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:3622136/35‑1 (MQ=255) aGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATa < 1:3125829/71‑1 (MQ=255) aGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATa < 1:1801403/71‑1 (MQ=255) gTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGgcag < 1:606366/54‑1 (MQ=255) gTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGgcag < 1:1934067/54‑1 (MQ=255) tcatcaTCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGcc > 1:3380208/1‑71 (MQ=255) tcatcaTCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGcc > 1:3384441/1‑71 (MQ=255) tcatcTCTGAGGTCATCGGCGGGACGATAACGCGATa < 1:2970391/37‑1 (MQ=255) tcatcTCTGAGGTCATCGGCGGGACGATAACGCGATa < 1:3355649/37‑1 (MQ=255) tcatcTCTGAGGTCATCGGCGGGACGATAACGCGATa < 1:2758626/37‑1 (MQ=255) atcTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTAt > 1:3164010/1‑57 (MQ=255) tctcTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATc < 1:2535083/57‑1 (MQ=255) tctcTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCgg > 1:2815153/1‑70 (MQ=255) tctGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAg > 1:3375907/1‑71 (MQ=255) ctGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTa > 1:815077/1‑69 (MQ=255) ctGAGGTCATCGGCGGGACGATAAAGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTa > 1:2698220/1‑69 (MQ=255) | CTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAG > W3110S.gb/686501‑686638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |