| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 701,210 | A→G | 18.2% | A269A (GCT→GCC) | nagC ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 701,210 | 0 | A | G | 18.2% | 91.3 / 13.6 | 44 | A269A (GCT→GCC) | nagC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (16/20); new base G (5/3); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGC > W3110S.gb/701142‑701280 | aGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGc < 1:3071297/71‑1 (MQ=255) ggCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGa > 1:137996/1‑70 (MQ=255) ggCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGa > 1:206907/1‑70 (MQ=255) gCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATa < 1:3286573/71‑1 (MQ=255) gCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATa < 1:54008/71‑1 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCATTGGCGGCGATa > 1:2364428/1‑57 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCATTGGCGGCGATAg > 1:3327231/1‑58 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCATTGGCGGCGATAg > 1:3322712/1‑58 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCATTGGCGGCGATAg > 1:2574518/1‑58 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCATTGGCGGCGATAg > 1:2448050/1‑58 (MQ=255) ccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCaaa < 1:967092/70‑1 (MQ=255) cTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGt < 1:1310696/54‑1 (MQ=255) cTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCaa < 1:1748948/68‑1 (MQ=255) tGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAg < 1:547753/63‑1 (MQ=255) tGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCaa < 1:3087723/67‑1 (MQ=255) cTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGAt < 1:2349899/48‑1 (MQ=255) aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGc < 1:427992/70‑1 (MQ=255) aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGc < 1:3304949/70‑1 (MQ=255) aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGc < 1:1016942/70‑1 (MQ=255) aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGc < 1:304476/70‑1 (MQ=255) aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGCTGCCGc < 1:492044/70‑1 (MQ=255) aGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAg > 1:2099501/1‑60 (MQ=255) aGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTg > 1:3189489/1‑71 (MQ=255) ttCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTcc > 1:30193/1‑45 (MQ=255) ttCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGAAAGTTTCCAGGCAGCCAAAGTTGCCGCAGTgg > 1:3675562/1‑69 (MQ=255) tCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAg > 1:3798019/1‑71 (MQ=255) acCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCa > 1:2043156/1‑40 (MQ=255) acCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCa > 1:709455/1‑40 (MQ=255) cGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCa > 1:1438726/1‑41 (MQ=255) gttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCAcc > 1:2739123/1‑70 (MQ=255) tgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCa < 1:3720863/70‑1 (MQ=255) ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGc > 1:540461/1‑70 (MQ=255) tCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCgg > 1:1668865/1‑71 (MQ=255) gCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCgg < 1:3810597/62‑1 (MQ=255) cattGGCGGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa < 1:287546/69‑1 (MQ=255) cattGGCGGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa < 1:2596305/69‑1 (MQ=255) cattGGCGGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa < 1:2334773/69‑1 (MQ=255) gTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa < 1:2350999/71‑1 (MQ=255) gTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa < 1:1301376/71‑1 (MQ=255) ttGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGt < 1:626275/60‑1 (MQ=255) ggCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAatat > 1:137110/1‑71 (MQ=255) gCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAatat < 1:1394981/70‑1 (MQ=255) gCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATg > 1:321807/1‑71 (MQ=255) gCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATg > 1:1136035/1‑71 (MQ=255) aGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGc > 1:154780/1‑71 (MQ=255) | AGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGC > W3110S.gb/701142‑701280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |