| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 726,688 | C→T | 14.8% | A257A (GCG→GCA) | kdpB ← | potassium translocating ATPase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 726,688 | 0 | C | T | 14.8% | 61.4 / 3.6 | 27 | A257A (GCG→GCA) | kdpB | potassium translocating ATPase, subunit B |
| Reads supporting (aligned to +/- strand): ref base C (10/13); new base T (2/2); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGC > W3110S.gb/726624‑726756 | tCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGccac > 1:2503654/1‑70 (MQ=255) ccGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAg > 1:542126/1‑71 (MQ=255) ccGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAg > 1:2814665/1‑71 (MQ=255) gCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTAc > 1:3666868/1‑71 (MQ=255) cGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGtt < 1:3590031/71‑1 (MQ=255) gATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTaa > 1:377104/1‑62 (MQ=255) gCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGTGCCACCAGTAcc < 1:1477928/60‑1 (MQ=255) gCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGTGCCACCAGTAcc < 1:1913224/60‑1 (MQ=255) gCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAg > 1:947880/1‑56 (MQ=255) cTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTAcc < 1:1554685/59‑1 (MQ=255) acaacaGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGt < 1:1846092/53‑1 (MQ=255) acaacaGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTg < 1:362822/69‑1 (MQ=255) acaacaGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGc < 1:888875/70‑1 (MQ=255) aacaGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCAt > 1:563068/1‑70 (MQ=255) ggCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt < 1:1524403/58‑1 (MQ=255) cAATAGTGGTTGGGATCAGACAGACCAGCAGTGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCAc > 1:17137/1‑71 (MQ=255) cAATAGTGGTTGGGATCAGACAGACCAGCAGTGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCAc > 1:784216/1‑71 (MQ=255) aTAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGt < 1:1146523/39‑1 (MQ=255) aTAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGt < 1:3565636/39‑1 (MQ=255) gTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTAcc > 1:2938936/1‑39 (MQ=255) tGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGaa < 1:2207299/71‑1 (MQ=255) tGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGaa < 1:1616003/71‑1 (MQ=255) tGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGaa < 1:1358298/71‑1 (MQ=255) ggATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGc < 1:1160681/71‑1 (MQ=255) gATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTg < 1:1120936/40‑1 (MQ=255) tCAGACAGACCAGCAGCGCCACCAGTACCGTTACg > 1:2845602/1‑35 (MQ=255) cagcagCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCgg > 1:2549722/1‑71 (MQ=255) gcagcGCCACCAGTACCGTTACGCTGACTGCATTa > 1:359054/1‑35 (MQ=255) gcagcGCCACCAGTACCGTTACGCTGACTGCATTa > 1:2586249/1‑35 (MQ=255) gcagcGCCACCAGTACCGTTACGCTGACTGCATTACCGACCCACGCGGAAAACGGCCACAGCGTGGCGGtt < 1:1557089/71‑1 (MQ=255) agcGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGc < 1:3732610/71‑1 (MQ=255) | TCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGC > W3110S.gb/726624‑726756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |