| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 740,339 | T→G | 7.3% | P137P (CCT→CCG) | phr → | deoxyribodipyrimidine photolyase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 740,339 | 0 | T | G | 7.3% | 130.1 / 5.2 | 55 | P137P (CCT→CCG) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base T (22/29); new base G (2/2); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
ATGTGGAAGTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAAC > W3110S.gb/740269‑740409 | aTGTGGAAGTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCt < 1:3214158/71‑1 (MQ=255) gAAGTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTg > 1:562549/1‑67 (MQ=255) gAAGTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGgcg < 1:3226995/70‑1 (MQ=255) aGTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCgg < 1:545677/71‑1 (MQ=255) gTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGt < 1:3221457/71‑1 (MQ=255) gTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGGGATCCTGCCGCCTGGCGCGGt < 1:3716980/71‑1 (MQ=255) tGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGTCGCCTGGCGCGGtga > 1:3112495/1‑71 (MQ=255) tGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGtga > 1:2989018/1‑71 (MQ=255) tGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGtga > 1:3433089/1‑71 (MQ=255) aaaGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGtgatg < 1:489395/71‑1 (MQ=255) agagCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGAc < 1:34667/71‑1 (MQ=255) agagCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGAc < 1:325043/71‑1 (MQ=255) agagCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGAc < 1:3386631/71‑1 (MQ=255) agagCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGAc < 1:3425575/71‑1 (MQ=255) agCGCTGCGTAACGTGGTGTCTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGtga > 1:2366021/1‑65 (MQ=255) tGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCGTGGGCGGTGATGACTggc > 1:3826547/1‑67 (MQ=255) tGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCGGGGGCGGTGATGACTggc > 1:1420604/1‑67 (MQ=255) cGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAAt < 1:1974475/69‑1 (MQ=255) cGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAAt < 1:2144321/69‑1 (MQ=255) aCGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTa < 1:3002777/63‑1 (MQ=255) aCGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACga < 1:112297/70‑1 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGTTAATCAc > 1:3154050/1‑67 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGTTAATCAc > 1:3149627/1‑67 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCAc > 1:3557174/1‑67 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCAc > 1:3086921/1‑67 (MQ=255) tgAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACa < 1:380179/68‑1 (MQ=255) gAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGtg < 1:900707/42‑1 (MQ=255) aaGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTc > 1:1759305/1‑71 (MQ=255) aaGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATCACCGGTAATCACGAGATGTACAAAGTc < 1:1603020/71‑1 (MQ=255) aaGGATTTGATGACAGCGTGATCCTGCCGCCGGGGGCGGTGATGACTGGCAATCatg < 1:1813783/57‑3 (MQ=37) aaGGATTTGATGACAGCGTGATCCTGCCGCCGCGGGCGGTGATGACTGGCAATCatg < 1:304951/57‑3 (MQ=255) aGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCt > 1:2044585/1‑71 (MQ=255) ggttttGATGACAGCGTGCTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACTAAGTCtt > 1:1215023/4‑71 (MQ=255) ggATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCtt < 1:569343/71‑1 (MQ=255) ggATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCtt > 1:2986453/1‑71 (MQ=255) aTTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCtt < 1:1915733/69‑1 (MQ=255) tttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:1141119/1‑70 (MQ=255) tttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:1145700/1‑70 (MQ=255) ttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACg > 1:1222540/1‑71 (MQ=255) ttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACg > 1:2390418/1‑71 (MQ=255) tgatgaCAGCGTGATCCTTCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGc > 1:3537802/1‑71 (MQ=255) tgatgaCAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGc > 1:2205957/1‑71 (MQ=255) gaCAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCttt > 1:1913154/1‑71 (MQ=255) aGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTaa < 1:2131144/71‑1 (MQ=255) gCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAg < 1:2385090/71‑1 (MQ=255) gCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAg < 1:3119577/71‑1 (MQ=255) cGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGa < 1:1969111/71‑1 (MQ=255) gTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGaa > 1:2024887/1‑71 (MQ=255) aTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACg < 1:1570745/37‑1 (MQ=255) aTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACa < 1:3036205/46‑1 (MQ=255) aTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACa < 1:468215/46‑1 (MQ=255) tCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACg < 1:3232452/36‑1 (MQ=255) ccTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCt > 1:1373180/1‑71 (MQ=255) cTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGc < 1:843578/68‑1 (MQ=255) tGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGt < 1:535084/46‑1 (MQ=255) gccTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGa < 1:3339362/71‑1 (MQ=255) tGGCGCGGTGATGACCTGTAATCACGAGATGTACAAAGTCTTTa > 1:1227035/1‑44 (MQ=255) tGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:3673319/1‑44 (MQ=255) tGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:80085/1‑44 (MQ=255) tGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAAc > 1:768015/1‑71 (MQ=255) | ATGTGGAAGTTGAAAGAGCGCTGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAAC > W3110S.gb/740269‑740409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |