| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 740,354 | C→T | 7.4% | T142T (ACC→ACT) | phr → | deoxyribodipyrimidine photolyase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 740,354 | 0 | C | T | 7.4% | 157.3 / 2.3 | 54 | T142T (ACC→ACT) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (23/27); new base T (2/2); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
TGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGG > W3110S.gb/740290‑740422 | tGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCGTGGGCGGTGATGACTggc > 1:3826547/1‑67 (MQ=255)tGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCGGGGGCGGTGATGACTggc > 1:1420604/1‑67 (MQ=255) cGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAAt < 1:2144321/69‑1 (MQ=255) cGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAAt < 1:1974475/69‑1 (MQ=255) aCGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTa < 1:3002777/63‑1 (MQ=255) aCGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACga < 1:112297/70‑1 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGTTAATCAc > 1:3154050/1‑67 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGTTAATCAc > 1:3149627/1‑67 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCAc > 1:3557174/1‑67 (MQ=255) cGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCAc > 1:3086921/1‑67 (MQ=255) tgAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACa < 1:380179/68‑1 (MQ=255) aaGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTc > 1:1759305/1‑71 (MQ=255) aaGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATCACCGGTAATCACGAGATGTACAAAGTc < 1:1603020/71‑1 (MQ=255) aaGGATTTGATGACAGCGTGATCCTGCCGCCGGGGGCGGTGATGACTGGCAATCatg < 1:1813783/57‑3 (MQ=37) aaGGATTTGATGACAGCGTGATCCTGCCGCCGCGGGCGGTGATGACTGGCAATCatg < 1:304951/57‑3 (MQ=255) aGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCt > 1:2044585/1‑71 (MQ=255) ggttttGATGACAGCGTGCTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACTAAGTCtt > 1:1215023/4‑71 (MQ=255) ggATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCtt < 1:569343/71‑1 (MQ=255) ggATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCtt > 1:2986453/1‑71 (MQ=255) aTTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCtt < 1:1915733/69‑1 (MQ=255) tttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:1145700/1‑70 (MQ=255) tttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:1141119/1‑70 (MQ=255) ttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACg > 1:1222540/1‑71 (MQ=255) ttGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACg > 1:2390418/1‑71 (MQ=255) tgatgaCAGCGTGATCCTTCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGc > 1:3537802/1‑71 (MQ=255) tgatgaCAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGc > 1:2205957/1‑71 (MQ=255) gaCAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCttt > 1:1913154/1‑71 (MQ=255) aGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTaa < 1:2131144/71‑1 (MQ=255) gCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAg < 1:3119577/71‑1 (MQ=255) gCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAg < 1:2385090/71‑1 (MQ=255) cGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGa < 1:1969111/71‑1 (MQ=255) gTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGaa > 1:2024887/1‑71 (MQ=255) aTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACg < 1:1570745/37‑1 (MQ=255) aTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACa < 1:3036205/46‑1 (MQ=255) aTCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACa < 1:468215/46‑1 (MQ=255) tCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACg < 1:3232452/36‑1 (MQ=255) ccTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCt > 1:1373180/1‑71 (MQ=255) cTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGc < 1:843578/68‑1 (MQ=255) tGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGt < 1:535084/46‑1 (MQ=255) gccTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGa < 1:3339362/71‑1 (MQ=255) tGGCGCGGTGATGACCTGTAATCACGAGATGTACAAAGTCTTTa > 1:1227035/1‑44 (MQ=255) tGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:80085/1‑44 (MQ=255) tGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTa > 1:3673319/1‑44 (MQ=255) tGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAAc > 1:768015/1‑71 (MQ=255) gcgcGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAAc < 1:3520640/69‑1 (MQ=255) gcgcGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAAc < 1:1175795/69‑1 (MQ=255) gcgcGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACgg > 1:133318/1‑71 (MQ=255) tgatgaCCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAg < 1:2178851/47‑1 (MQ=255) tgatgaCCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAg < 1:585089/47‑1 (MQ=255) tgatgaCCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCgg > 1:3587410/1‑71 (MQ=255) atgaCCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCtgg < 1:2279439/70‑1 (MQ=255) atgaCCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCggg < 1:1047590/70‑1 (MQ=255) atgaCCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCggg < 1:2723328/70‑1 (MQ=255) aCCTGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAgg < 1:1828719/71‑1 (MQ=255) ccGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAgg < 1:1881810/70‑1 (MQ=255) cGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTaa > 1:2173142/1‑39 (MQ=255) | TGCGTAACGTGGTGTGTGAAGGATTTGATGACAGCGTGATCCTGCCGCCTGGCGCGGTGATGACCGGTAATCACGAGATGTACAAAGTCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGG > W3110S.gb/740290‑740422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |