| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 775,925 | C→T | 11.3% | N117N (AAC→AAT) | tolQ → | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 775,925 | 0 | C | T | 11.3% | 116.8 / 5.3 | 44 | N117N (AAC→AAT) | tolQ | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base C (26/13); new base T (2/3); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
ATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTA > W3110S.gb/775864‑775990 | aTAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACtt > 1:914668/1‑71 (MQ=255)aTAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACtt > 1:3655038/1‑71 (MQ=255)aTAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACtt > 1:1130348/1‑71 (MQ=255) aGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTg > 1:599846/1‑70 (MQ=255) aGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTg > 1:1591106/1‑70 (MQ=255) aGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTg > 1:2733028/1‑70 (MQ=255) aGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTg > 1:587807/1‑70 (MQ=255) cgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGaaa > 1:2189071/1‑66 (MQ=255) cgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTg < 1:3723550/71‑1 (MQ=255) cgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTg > 1:757596/1‑71 (MQ=255) cgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTg > 1:2266147/1‑71 (MQ=255) cgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTg > 1:685589/1‑71 (MQ=255) gcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATTAAc > 1:3252010/1‑52 (MQ=255) ccGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGa < 1:1256650/71‑1 (MQ=255) cGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAAc < 1:1883843/57‑1 (MQ=255) ggAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAAc < 1:2224981/56‑1 (MQ=255) gAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACtt > 1:2250345/1‑57 (MQ=255) gAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACtt > 1:568252/1‑57 (MQ=255) gAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACtt > 1:416458/1‑57 (MQ=255) aaGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACg < 1:2517166/71‑1 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTTAACTTGAAAAt < 1:1170527/54‑1 (MQ=255) gTGGAAGGGGCGTCCCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAAt < 1:3270425/54‑1 (MQ=255) gAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGaaa > 1:2953122/1‑58 (MQ=255) gAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGaaa > 1:664826/1‑58 (MQ=255) gAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGtt < 1:525437/71‑1 (MQ=255) ggggCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTcc > 1:1650808/1‑70 (MQ=255) ggCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCg < 1:1006763/64‑1 (MQ=255) tCGCGTGCTATGCGTATCTCCATGAACCGTTAACTTGAAAATCTGGa < 1:1425626/47‑1 (MQ=255) tCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTAc > 1:3228372/1‑71 (MQ=255) tCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTAc > 1:764982/1‑71 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTc > 1:2201320/1‑61 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCgg > 1:1887679/1‑66 (MQ=255) cgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:2754076/71‑1 (MQ=255) tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:3015056/70‑1 (MQ=255) tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:3606344/1‑70 (MQ=255) tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:3118004/1‑70 (MQ=255) tATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATCa < 1:3567472/71‑1 (MQ=255) tATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATCa < 1:3282476/71‑1 (MQ=255) tATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATCa < 1:1038000/71‑1 (MQ=255) tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAg > 1:1977900/1‑68 (MQ=255) tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGccc > 1:2362009/1‑71 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGt > 1:3845927/1‑41 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:3255098/1‑70 (MQ=255) aTGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGta < 1:1617961/71‑1 (MQ=255) aaCCGTGAACTTGAAAATCTGGAAACGCACATTCCGtt > 1:3527176/1‑38 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:3829040/54‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:1668835/54‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:1385745/54‑1 (MQ=255) | ATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTA > W3110S.gb/775864‑775990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |