| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 775,958 | G→T | 10.3% | P128P (CCG→CCT) | tolQ → | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 775,958 | 0 | G | T | 10.3% | 146.0 / 2.8 | 48 | P128P (CCG→CCT) | tolQ | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base G (19/24); new base T (2/3); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCAC > W3110S.gb/775890‑776027 | gAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGtt < 1:525437/71‑1 (MQ=255) ggggCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTcc > 1:1650808/1‑70 (MQ=255) ggCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCg < 1:1006763/64‑1 (MQ=255) tCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTAc > 1:764982/1‑71 (MQ=255) tCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTAc > 1:3228372/1‑71 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTc > 1:2201320/1‑61 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCgg > 1:1887679/1‑66 (MQ=255) cgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:2754076/71‑1 (MQ=255) tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:3015056/70‑1 (MQ=255) tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:3606344/1‑70 (MQ=255) tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:3118004/1‑70 (MQ=255) tATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATCa < 1:1038000/71‑1 (MQ=255) tATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATCa < 1:3282476/71‑1 (MQ=255) tATTTCCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATCa < 1:3567472/71‑1 (MQ=255) tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAg > 1:1977900/1‑68 (MQ=255) tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGccc > 1:2362009/1‑71 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGt > 1:3845927/1‑41 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:3255098/1‑70 (MQ=255) aTGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGta < 1:1617961/71‑1 (MQ=255) aaCCGTGAACTTGAAAATCTGGAAACGCACATTCCGtt > 1:3527176/1‑38 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:3829040/54‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:1668835/54‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc < 1:1385745/54‑1 (MQ=255) gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGt > 1:363043/1‑71 (MQ=255) aaCTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc < 1:3058461/40‑1 (MQ=255) aaCTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt > 1:3599059/1‑43 (MQ=255) aaCTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt > 1:1281431/1‑43 (MQ=255) tGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTatat > 1:2595615/1‑60 (MQ=255) gAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata > 1:976105/1‑58 (MQ=255) gAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTGTGTTTgg > 1:2974054/1‑71 (MQ=255) gAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGttt < 1:2697179/69‑1 (MQ=255) gAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTGTgg > 1:1463936/1‑71 (MQ=255) aaaaTCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTgg < 1:284905/70‑1 (MQ=255) aaTCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:3355382/55‑1 (MQ=255) aaTCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTTGTCTGttt < 1:3759364/66‑1 (MQ=255) aTCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACg < 1:1329221/71‑1 (MQ=255) tGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACgg < 1:3188432/69‑1 (MQ=255) tGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTc > 1:1818741/1‑71 (MQ=255) gAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCt < 1:878161/70‑1 (MQ=255) gAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCt < 1:2066994/70‑1 (MQ=255) cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTggggg < 1:1091028/71‑1 (MQ=255) cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTggggg < 1:2364160/71‑1 (MQ=255) cacaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGAt < 1:2075966/71‑1 (MQ=255) aTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGttt < 1:2212787/51‑1 (MQ=255) aTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGttt < 1:814828/51‑1 (MQ=255) ttCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCt < 1:2996272/60‑1 (MQ=255) ttCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATg < 1:3678976/71‑1 (MQ=255) ttCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATg < 1:26010/71‑1 (MQ=255) cGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCAc > 1:2451975/1‑71 (MQ=255) | GAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCAC > W3110S.gb/775890‑776027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |