| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 798,591 | A→T | 11.1% | I88N (ATT→AAT) | ybhA ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 798,591 | 0 | A | T | 11.1% | 113.6 / 7.3 | 45 | I88N (ATT→AAT) | ybhA | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (17/23); new base T (2/3); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
ATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCA > W3110S.gb/798519‑798661 | aTTGCATCATCGACATACATCAGACCGTGAATGTG‑‑GTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaa > 1:358010/1‑71 (MQ=255) catcatCGACATACATCAGACCGTGAATGTGGTGTTCATTCGGCATCTCAATGAGTTGCAGGGCTTtaat < 1:1070624/70‑1 (MQ=255) cGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACggg < 1:3365046/70‑1 (MQ=255) cGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACgg > 1:2186194/1‑69 (MQ=255) acatacatCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaat < 1:3390749/62‑1 (MQ=255) catCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGt > 1:2652800/1‑71 (MQ=255) catCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGt < 1:1928466/71‑1 (MQ=255) catCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGt > 1:1922168/1‑71 (MQ=255) catCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGt > 1:1545086/1‑71 (MQ=255) tCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTcc > 1:2432910/1‑71 (MQ=255) tCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTcc < 1:328674/71‑1 (MQ=255) cAGACCGTGAATGTGGTGTTCATACAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGt > 1:3673737/1‑68 (MQ=255) aGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGc > 1:1328599/1‑71 (MQ=255) gACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCt < 1:2450329/71‑1 (MQ=255) gAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTATTAACGGGCATTGGGTCCGCTTCCAg > 1:364282/1‑70 (MQ=255) gAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTATTAACGGGCATTGGGTCCGCTTCCAg > 1:2404688/1‑70 (MQ=255) aaTGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAg < 1:533983/69‑1 (MQ=255) aaTGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGc < 1:1818462/70‑1 (MQ=255) aaTGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGc < 1:3844521/70‑1 (MQ=255) tgtgGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACg > 1:2327319/1‑71 (MQ=255) tggtgTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGt > 1:570318/1‑71 (MQ=255) tgtTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaataa < 1:2195737/40‑1 (MQ=255) ttcattcaGCATCTCAATGAGTTGCAGGGCTTtaa < 1:2853626/35‑1 (MQ=255) ttcattcaGCATCTCAATGAGTTGCAGGGCTTTATTAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTg < 1:754510/71‑1 (MQ=255) ttcattcaGCATCTCAATGAGTTGCAGGGCTTTATTAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTg < 1:2438173/71‑1 (MQ=255) ttaattcaGCATCTCAATGAGTTGCAGGGCTTTATTAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTg < 1:1217155/68‑1 (MQ=255) attcaGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCa < 1:1705136/56‑1 (MQ=255) caGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGa > 1:3307370/1‑70 (MQ=255) tcAATGAGTTGCAGGGCTTTAATAACGGGCATTgg > 1:2730436/1‑35 (MQ=255) tcAATGAGTTGCAGGGCTTTAATAACGGGCATTgg > 1:2066335/1‑35 (MQ=255) tcAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATa > 1:217803/1‑71 (MQ=255) tcAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATa > 1:3409973/1‑71 (MQ=255) aaTGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATTATAATCATACa < 1:1742625/71‑1 (MQ=255) aaTGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCa < 1:3687988/67‑1 (MQ=255) aaTGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACa < 1:3024065/71‑1 (MQ=255) aaTGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACa < 1:3788964/71‑1 (MQ=255) tGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACaaa < 1:1003801/71‑1 (MQ=255) tGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACaaa < 1:3292195/71‑1 (MQ=255) aGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCa > 1:3263668/1‑43 (MQ=255) aGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAAt < 1:465818/70‑1 (MQ=255) aGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAAt < 1:713363/70‑1 (MQ=255) aGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAAt < 1:2607744/70‑1 (MQ=255) gTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAg < 1:2136577/71‑1 (MQ=255) gTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAg < 1:2447467/71‑1 (MQ=255) aGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGc > 1:3294465/1‑70 (MQ=255) aGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGc > 1:544089/1‑70 (MQ=255) ggCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACa < 1:3424989/59‑1 (MQ=255) ggCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACa < 1:3806592/59‑1 (MQ=255) ggCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACa < 1:526080/59‑1 (MQ=255) cTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTa < 1:1297050/71‑1 (MQ=255) aataaCGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATAc > 1:3733740/1‑52 (MQ=255) ataaCGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCa < 1:3565948/71‑1 (MQ=255) | ATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCA > W3110S.gb/798519‑798661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |