| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 912,872 | T→A | 7.7% | A455A (GCA→GCT) | hcp ← | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 912,872 | 0 | T | A | 7.7% | 138.1 / 4.0 | 52 | A455A (GCA→GCT) | hcp | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Reads supporting (aligned to +/- strand): ref base T (30/18); new base A (2/2); total (32/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
CAGCCCAGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTAC > W3110S.gb/912807‑912936 | cAGCCCAGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATc < 1:700022/71‑1 (MQ=255) cccAGTTTCTCTGCCAGCGTTACCGCCAGAATAATTGCTGAGTAAGCATCGTTACACTGACCAGCATCTAc < 1:3818234/71‑1 (MQ=255) cccAGTTTCTCTGCCAGCGTTACCGCCAGAATAATTGCTGAGTAAGCATCGTTACACTGACCAGCATCTAc < 1:1907604/71‑1 (MQ=255) cccAGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTAc < 1:642188/71‑1 (MQ=255) ccAGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTAc < 1:1073011/70‑1 (MQ=255) cAGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTAc > 1:2965863/1‑69 (MQ=255) aGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTAc > 1:2490347/1‑68 (MQ=255) ttCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGAcg > 1:660504/1‑71 (MQ=255) tctcTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGAcgc > 1:1441331/1‑71 (MQ=255) tctcTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGAcgc > 1:1810274/1‑71 (MQ=255) ctctGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGAcgcg > 1:3484196/1‑71 (MQ=255) ctctGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGAcgcg > 1:2581759/1‑71 (MQ=255) tGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCgg > 1:2364219/1‑69 (MQ=255) ccAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAg < 1:795755/70‑1 (MQ=255) cAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGAc > 1:1896070/1‑71 (MQ=255) cAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGAc > 1:1346845/1‑71 (MQ=255) tGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTc > 1:142846/1‑70 (MQ=255) tGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTc > 1:3546639/1‑70 (MQ=255) tGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTc < 1:2787180/70‑1 (MQ=255) gACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGC‑GCAGACCTTCGa > 1:554944/1‑70 (MQ=255) aCAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGa < 1:924185/70‑1 (MQ=255) ataatCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCa > 1:1046936/1‑52 (MQ=255) ataatCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCa > 1:538957/1‑52 (MQ=255) ataatCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCaaa > 1:605747/1‑71 (MQ=255) taatCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAAc > 1:800709/1‑71 (MQ=255) atCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTc < 1:401006/56‑1 (MQ=255) atCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACt > 1:470968/1‑70 (MQ=255) atCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACt > 1:2948252/1‑70 (MQ=255) atCGCTGAGTAAGCATCGTTACATTGACATGAATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACt > 1:3138721/1‑70 (MQ=255) gCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAg > 1:2474305/1‑71 (MQ=255) gCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAg > 1:2346518/1‑71 (MQ=255) gAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGttt > 1:975018/1‑71 (MQ=255) aGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGttt < 1:3575968/70‑1 (MQ=255) aGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGttt < 1:2599789/70‑1 (MQ=255) gTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAg < 1:2612176/66‑1 (MQ=255) gTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAg < 1:2581119/66‑1 (MQ=255) tAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGtt < 1:1572786/71‑1 (MQ=255) aaGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAATTTTGtt < 1:495843/70‑1 (MQ=255) aTCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGc > 1:1578236/1‑71 (MQ=255) tCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGa > 1:2905760/1‑43 (MQ=255) tCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCa < 1:1416761/57‑1 (MQ=255) cGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGa > 1:3544907/1‑71 (MQ=255) gTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCaaa > 1:3083705/1‑51 (MQ=255) gTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCaaa > 1:2851406/1‑51 (MQ=255) gTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCaaa > 1:2284924/1‑51 (MQ=255) gTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGat < 1:2502936/71‑1 (MQ=255) aCATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGatat < 1:910689/70‑1 (MQ=255) aCATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATAtt > 1:1043422/1‑71 (MQ=255) cATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATAtt < 1:3291917/70‑1 (MQ=255) gACCAGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGttt > 1:1836102/1‑52 (MQ=255) gACCAGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGttt > 1:2313983/1‑52 (MQ=255) cTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATAtt < 1:2820737/63‑1 (MQ=255) tGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTAc < 1:3213124/65‑1 (MQ=255) | CAGCCCAGTTTCTCTGCCAGAGTGACAGCCAGAATAATCGCTGAGTAAGCATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTAC > W3110S.gb/912807‑912936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |