Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 944,111 T→C 14.5% V92V (GTT→GTC dmsB → dimethyl sulfoxide reductase, anaerobic, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb944,1110TC14.5% 151.1 / 16.1 62V92V (GTT→GTCdmsBdimethyl sulfoxide reductase, anaerobic, subunit B
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base C (5/4);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

CTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATGGCGCACCGCA  >  W3110S.gb/944042‑944179
                                                                     |                                                                    
cTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:332089/71‑1 (MQ=255)
cTGCGAAGATCCGGCTTGTACTAAAGTCTCCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:2055592/71‑1 (MQ=255)
        aTCCGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:2152288/63‑1 (MQ=255)
        aTCCGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgtt                                                                      <  1:2837561/62‑1 (MQ=255)
         tCCGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGa                                                              >  1:2774955/1‑69 (MQ=255)
         tCCGGCTTGTACTAAAGTCTGCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGa                                                           <  1:3206272/71‑1 (MQ=255)
          ccGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATg                                                            >  1:2191147/1‑70 (MQ=255)
          ccGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATg                                                            >  1:2640380/1‑70 (MQ=255)
           cGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGaa                                                          <  1:2734759/71‑1 (MQ=255)
           cGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGaa                                                          <  1:2262587/71‑1 (MQ=255)
           cGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGaa                                                          <  1:1849787/71‑1 (MQ=255)
             gCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGa                                                        <  1:3178161/71‑1 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttt                                                                     <  1:3537799/53‑2 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:1369420/53‑1 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:3068187/53‑1 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:51909/53‑1 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:2738129/53‑1 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGAtgtgt                                                   <  1:3384957/71‑1 (MQ=255)
                  tACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGAtgtgt                                                   <  1:2885780/71‑1 (MQ=255)
                    cTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGa                                                        >  1:143366/1‑64 (MQ=255)
                    cTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGc                                                 <  1:1655112/71‑1 (MQ=255)
                    cTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGc                                                 <  1:3660828/71‑1 (MQ=255)
                    cTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGc                                                 <  1:303411/71‑1 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCTATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAt                                               <  1:2860338/70‑1 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAtt                                              >  1:2183527/1‑71 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAtt                                              >  1:2052248/1‑71 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAtt                                              >  1:1899140/1‑71 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAtt                                              >  1:3690276/1‑71 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAt                                               <  1:775399/70‑1 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAt                                               <  1:2167533/70‑1 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAt                                               <  1:1799326/70‑1 (MQ=255)
                       aaGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAACATGTGTGCAtt                                              >  1:1543131/1‑71 (MQ=255)
                        aGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTg                                             <  1:3806056/71‑1 (MQ=255)
                        aGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTg                                             <  1:3567171/71‑1 (MQ=255)
                          tCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTttgttg                                                                     <  1:644086/45‑1 (MQ=255)
                              cccGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCt                                          <  1:2715880/68‑1 (MQ=255)
                                  aGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTgtc                                       >  1:2060763/1‑65 (MQ=18)
                                  aGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTgtc                                       >  1:3563770/1‑65 (MQ=18)
                                        gCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATg                                                            >  1:1452719/1‑40 (MQ=255)
                                        gCGATGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTGTCGTTACTGCCa                             >  1:2173544/1‑71 (MQ=17)
                                        gCGATGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTGTCGTTACTGCCa                             >  1:2690657/1‑71 (MQ=17)
                                        gCGATGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTGTCGTTACTGCCa                             >  1:2250799/1‑71 (MQ=17)
                                         cGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCAtt                                              >  1:1141521/1‑53 (MQ=255)
                                         cGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCa                             <  1:3597288/70‑1 (MQ=255)
                                         cGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCa                             <  1:1846706/70‑1 (MQ=255)
                                           aTGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCatat                          <  1:1233253/71‑1 (MQ=255)
                                            tGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATg                                                            <  1:2284950/36‑1 (MQ=255)
                                            tGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTgtc                                       <  1:3324783/57‑3 (MQ=17)
                                            tGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTgtc                                       <  1:1145150/57‑3 (MQ=17)
                                            tGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTgtc                                       <  1:1732587/57‑3 (MQ=17)
                                            tGCATAAACGTGAAGATGGTTTTGTCGTTGTCGATGAAGATGTGTGTATTGGCTgtc                                       <  1:2227992/57‑3 (MQ=17)
                                               aTAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCt                      >  1:560840/1‑71 (MQ=255)
                                                tAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCa                                                <  1:1521341/44‑1 (MQ=255)
                                                  aaCGTGAAGATGGTTTTGTTGTGGTCGATGAAGAt                                                       >  1:1907226/1‑35 (MQ=255)
                                                  aaCGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCa                                                >  1:3841856/1‑42 (MQ=255)
                                                  aaCGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCa                                                >  1:1137680/1‑42 (MQ=255)
                                                  aaCGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCa                                                >  1:2283934/1‑42 (MQ=255)
                                                  aaCGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTAc                                  >  1:866267/1‑56 (MQ=255)
                                                        aaGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCa                                                >  1:2432478/1‑36 (MQ=255)
                                                        aaGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCa                                                >  1:3650952/1‑36 (MQ=255)
                                                          gATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATgg           >  1:3249584/1‑71 (MQ=255)
                                                             ggTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCa                             >  1:1475356/1‑50 (MQ=255)
                                                             ggTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATGgcg         >  1:3015770/1‑70 (MQ=255)
                                                             ggTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATGgcg         >  1:1237220/1‑70 (MQ=255)
                                                             ggTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATGgcg         >  1:1315829/1‑70 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCt                                    <  1:1873088/41‑1 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCt                                    <  1:1302892/41‑1 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCt                                    <  1:2288663/41‑1 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCt                                    <  1:66243/41‑1 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCt                                    <  1:1000568/41‑1 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGt               <  1:2056131/62‑1 (MQ=255)
                                                               ttttGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGt               <  1:3560681/62‑1 (MQ=255)
                                                                 ttgtcgttgtcgATGAAGATGTGTGTATTGGCTGTCGTTACTGCCATATTGCCTGCCCGTATGgcgc        >  1:1499577/1‑67 (MQ=255)
                                                                 ttgtcgttgtcgATGAAGATGTGTGTATTGGCTGTCGTTACTGCCATATTGCCTGCCCGTATGgcgc        >  1:1876394/1‑67 (MQ=255)
                                                                    ttgtGGTCTATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATGGCGCACCGCa  <  1:399976/70‑1 (MQ=255)
                                                                     |                                                                    
CTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCGAGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATTGGCTGCCGCTACTGCCATATGGCTTGCCCGTATGGCGCACCGCA  >  W3110S.gb/944042‑944179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: