Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,054,525 T→G 11.9% L692L (CTA→CTC torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,054,5250TG11.9% 82.9 / 9.0 42L692L (CTA→CTCtorShybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base T (23/14);  new base G (3/2);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGATTGACTGTTTTTGG  >  W3110S.gb/1054459‑1054593
                                                                  |                                                                    
ggCATTTCCTACTGCAACGACCTGCGCACCACTGGTGTTCAGCATTTCGACAGTAATTCGCTGGGTGAGCg                                                                  <  1:2719829/71‑1 (MQ=255)
ggCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCg                                                                  >  1:1082230/1‑71 (MQ=255)
 gCATTTCCTACTGCAACGACCTGCGCACCACTGGTGTTCAGCATTTCGACAGTAATTCGCTGGGTGAGCg                                                                  <  1:881321/70‑1 (MQ=255)
    ttGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGtt                                                                      <  1:1976549/63‑1 (MQ=255)
      gCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGtt                                                                      >  1:1570003/1‑61 (MQ=255)
      gCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTAt                                                            >  1:3240171/1‑71 (MQ=255)
       ccAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTAt                                                            <  1:2519642/70‑1 (MQ=255)
          aCAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTc                                                        <  1:648896/71‑1 (MQ=255)
          aCAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTc                                                        <  1:709389/71‑1 (MQ=255)
            aGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCggg                                                                >  1:2159031/1‑61 (MQ=255)
            aGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCg                                                                  >  1:177336/1‑59 (MQ=255)
            aGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCaa                                                      <  1:3544955/71‑1 (MQ=255)
                 caaTCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTaa                                                  >  1:2775949/1‑70 (MQ=255)
                   aTCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAg                                               >  1:3088468/1‑71 (MQ=255)
                   aTCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAg                                               >  1:1045535/1‑71 (MQ=255)
                      tGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTc                                                        >  1:1719659/1‑59 (MQ=255)
                      tGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCaa                                                      >  1:518834/1‑61 (MQ=255)
                        cgcACCACTTGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCa                                             <  1:3618850/68‑1 (MQ=255)
                        cgcACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAAc                                          >  1:1415572/1‑71 (MQ=255)
                             cacTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTaaa                                     <  1:2259433/71‑1 (MQ=255)
                              acTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAAc                                    >  1:1833467/1‑71 (MQ=255)
                              acTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAAc                                    >  1:2092485/1‑71 (MQ=255)
                                 ggTTTTCAGCATCTCAATGGTAATTCGCTGGGTTa                                                                     >  1:3299517/1‑35 (MQ=255)
                                 ggTTTTCAGCATCTCAATGGTAATTCGCTGGGTTa                                                                     >  1:258008/1‑35 (MQ=255)
                                 ggTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGt                                 >  1:178637/1‑71 (MQ=255)
                                  gTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGt                                 >  1:1138272/1‑70 (MQ=255)
                                  gTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTc                                <  1:2007135/71‑1 (MQ=255)
                                        aGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACg                          >  1:3843878/1‑71 (MQ=255)
                                            tctcAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAAtt                                                    <  1:3447605/41‑1 (MQ=255)
                                              tcAATGGTAATTCGCTGGGTTAGCGGGTTATCTTc                                                        <  1:1085950/35‑1 (MQ=255)
                                                aaTGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCt                  <  1:891731/71‑1 (MQ=255)
                                                   agTAATTCGCTGGGTGAGCGGGTTATCTTCAATTAACAGCAAACGCAAATCGTCCAGACGTAcc                      >  1:2520325/2‑64 (MQ=37)
                                                   agTAATTCGCTGGGTGAGCGGGTTATCTTCAATTAACAGCAAACGCAAATCGTCCAGACGTAcc                      >  1:3107605/2‑64 (MQ=37)
                                                    gTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGAtt              <  1:320623/71‑1 (MQ=255)
                                                      aaTTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGATTGa            >  1:606813/1‑71 (MQ=255)
                                                        ttCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGAtt              <  1:1277461/67‑1 (MQ=255)
                                                        ttCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGAtt              <  1:3020801/67‑1 (MQ=255)
                                                          cGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCaa                              >  1:2141972/1‑49 (MQ=255)
                                                          cGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGa                            >  1:3717303/1‑51 (MQ=255)
                                                          cGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGa                            >  1:1983795/1‑51 (MQ=255)
                                                          cGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGa                            >  1:1549802/1‑51 (MQ=255)
                                                          cGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCAc                       >  1:1300568/1‑56 (MQ=255)
                                                            cttggTGAGCGGGTTATCTTCAATTAACAGCAAACGCAAATCGTCCAGACGTACCGCCTGATTGACTGttt      >  1:476479/4‑71 (MQ=255)
                                                                gTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGATTGACTGTTTTTgg  <  1:2110032/71‑1 (MQ=255)
                                                                 ttAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTc                                <  1:1628784/40‑1 (MQ=255)
                                                                 ttAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTc                                <  1:133896/40‑1 (MQ=255)
                                                                  |                                                                    
GGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGGGTTAGCGGGTTATCTTCAATTAACAGCAAACGTAAACCGTCAAGACGCACCGCCTGATTGACTGTTTTTGG  >  W3110S.gb/1054459‑1054593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: