| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,094,325 | A→G | 9.4% | N343S (AAT→AGT) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,094,325 | 0 | A | G | 9.4% | 135.0 / 5.4 | 53 | N343S (AAT→AGT) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (19/29); new base G (3/2); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
GACCACTTCAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTCTT > W3110S.gb/1094255‑1094394 | gACCACTTCAAATAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGaa > 1:1020103/1‑71 (MQ=255) cACTTCAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAatat < 1:685150/71‑1 (MQ=255) tCAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCAtt > 1:968629/1‑71 (MQ=255) tCAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCAtt > 1:1801468/1‑71 (MQ=255) cAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCa > 1:2223569/1‑68 (MQ=255) cAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCa > 1:2776653/1‑68 (MQ=255) cAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCa > 1:308498/1‑68 (MQ=255) cAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCa > 1:3666927/1‑68 (MQ=255) cAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTg > 1:3793978/1‑71 (MQ=255) aaaGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGa > 1:2035508/1‑70 (MQ=255) aaaGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGa > 1:3092937/1‑70 (MQ=255) gTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAg < 1:3302734/71‑1 (MQ=255) aaCGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCa < 1:1705091/70‑1 (MQ=255) aaCGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCa < 1:1906417/70‑1 (MQ=255) aaCGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTAAATATCATTGGGAAAAGCa < 1:510124/70‑1 (MQ=255) cGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCa < 1:765936/56‑1 (MQ=255) cGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCacac < 1:1651540/71‑1 (MQ=255) cGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATAc < 1:1094717/71‑1 (MQ=255) acCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAGTATCATTGGGAAAAGCATACg > 1:1042860/1‑68 (MQ=255) acCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAGTATCATTGGGAAAAGCATACg > 1:1622244/1‑68 (MQ=255) ccTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCa < 1:3544606/71‑1 (MQ=255) ggggCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTTAATATCATTGGGaaa > 1:3166051/1‑55 (MQ=255) gggCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGa < 1:1923872/71‑1 (MQ=255) aTCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAGTATCAt > 1:475360/1‑43 (MQ=255) tCCGTTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCttt < 1:1952505/71‑1 (MQ=255) tCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGAcc < 1:1511434/60‑1 (MQ=255) tCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGAcc < 1:935354/60‑1 (MQ=255) tCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCttt < 1:626388/71‑1 (MQ=255) ccGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCttt > 1:3642843/1‑70 (MQ=255) cGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCa < 1:1708465/59‑1 (MQ=255) cGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCttt > 1:1116878/1‑69 (MQ=255) gTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCttt < 1:1175742/67‑1 (MQ=255) gTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAgc < 1:438456/71‑1 (MQ=255) ttGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGc < 1:1740455/47‑1 (MQ=255) ttGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGc < 1:1710309/47‑1 (MQ=255) ttGGCGATCAGGTGATAAAAACAGTGGGGAATATCATTGGGAAAAGc < 1:425853/47‑1 (MQ=255) tGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAgcgc > 1:2847596/1‑71 (MQ=255) gCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAgcgcg < 1:724448/70‑1 (MQ=255) gCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAgcgcg < 1:535544/70‑1 (MQ=255) aTCAGGTGATAAAAACAGTGGTGAGTATCATTGGGAAAAGCATACGGCCAGATGACCTTTTTGCGCGCGTc < 1:3521520/71‑1 (MQ=255) aTCAGGTGATAAAAACAGTGGTGAGTATCATTGGGAAAAGCATACGGCCAGATGACCTTTTTGCGCGCGTc < 1:2483407/71‑1 (MQ=255) aGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTcggc > 1:494450/1‑71 (MQ=255) aTAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTc > 1:1872817/1‑63 (MQ=255) aaaaCAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGa > 1:2314648/1‑68 (MQ=255) aaaaCAGTGGTGAATATCATTGGGAAAAGCATACGACAAGATGACCTTTTAGCGCGCGTCGGCGGCGaa > 1:65765/1‑69 (MQ=255) cAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGttt < 1:939175/71‑1 (MQ=255) tgtgAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGtt < 1:283045/65‑1 (MQ=255) gtgAATATCATTGGGAAAAGCATACGACCAGATGACCttt < 1:2075991/40‑1 (MQ=255) gtgAATATCATTGGGAAAAGCATACGACCAGATGACCttt < 1:3026908/40‑1 (MQ=255) gtgAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAg > 1:2422627/1‑43 (MQ=255) tgAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTc < 1:657144/71‑1 (MQ=255) tgAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTc < 1:1251442/71‑1 (MQ=255) tgAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTc < 1:2670284/71‑1 (MQ=255) tgAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTc < 1:2215432/71‑1 (MQ=255) gAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTCt > 1:3615764/1‑71 (MQ=255) aaTATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTcggc < 1:2278759/51‑1 (MQ=255) aaTATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGtt < 1:1985585/62‑1 (MQ=255) atatCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTCtt < 1:366483/70‑1 (MQ=255) | GACCACTTCAAAAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATACGACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTCTT > W3110S.gb/1094255‑1094394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |