| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,101,395 | G→A | 10.2% | R177R (CGC→CGT) | csgG ← | outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,101,395 | 0 | G | A | 10.2% | 132.9 / 5.0 | 49 | R177R (CGC→CGT) | csgG | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (23/21); new base A (2/3); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
TCATAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCTTGCCCC > W3110S.gb/1101327‑1101463 | tCATAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGAcgcg > 1:3271864/1‑71 (MQ=255) cATAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGAcgcg < 1:1600599/70‑1 (MQ=255) cATAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGAcgc < 1:48599/69‑1 (MQ=255) aTAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCa < 1:1902674/71‑1 (MQ=255) aTAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCa < 1:1762179/71‑1 (MQ=255) aaaGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGCCGCGCAGGt > 1:1206657/1‑69 (MQ=255) aaGTATCGTCTTACTGGTGTTCACCGAGGAGAGGATCTCACCGGTACTCACATTGACGACACGCAGGTTCa > 1:2407433/1‑71 (MQ=255) aaGTATCGTCTTACTGGTGTTCACCGAGGAGAGGATCTCACCGGTACTCACATTGACGACACGCAGGTTCa > 1:2064372/1‑71 (MQ=255) gTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACg > 1:1569429/1‑71 (MQ=255) tATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAg > 1:3424922/1‑63 (MQ=255) aTCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACgg > 1:1965651/1‑70 (MQ=255) gTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAg > 1:2889059/1‑59 (MQ=255) tGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCa < 1:1690788/62‑1 (MQ=255) gtgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCaa > 1:3448478/1‑61 (MQ=255) gtgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAAt > 1:170027/1‑62 (MQ=255) gtgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGa > 1:3017996/1‑66 (MQ=255) gtgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCg < 1:1745874/69‑1 (MQ=255) tgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGc > 1:464098/1‑71 (MQ=255) tgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGc > 1:327484/1‑71 (MQ=255) gtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCt > 1:1452404/1‑71 (MQ=255) ttCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCt < 1:1702099/70‑1 (MQ=255) cACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCa < 1:85671/50‑1 (MQ=255) cACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGAt > 1:3664889/1‑62 (MQ=255) cACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTgg > 1:910932/1‑70 (MQ=255) cACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTgg > 1:2013074/1‑70 (MQ=255) aCCGAAGAAAGGATCTCGCCGGTACTCACATTGACGCCGCGCAGGTTCACGGCAATCTGATCGAGCTGGTa > 1:2606300/1‑71 (MQ=255) aCCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGt < 1:2652531/70‑1 (MQ=255) gaagaaAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGt < 1:1792847/67‑1 (MQ=255) gaagaaAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGt < 1:1501965/67‑1 (MQ=255) aagaaAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGTCAATCTg < 1:3511222/55‑1 (MQ=255) aagaaAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTg < 1:2656628/55‑1 (MQ=255) aaGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGa < 1:1988221/56‑1 (MQ=255) aGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTc < 1:1201106/71‑1 (MQ=255) aGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTc < 1:2199198/71‑1 (MQ=255) ggATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCgtgt > 1:1470246/1‑69 (MQ=255) ggATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCgtgt > 1:2033269/1‑69 (MQ=255) tctcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCa > 1:1572147/1‑71 (MQ=255) gTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGt < 1:2412324/49‑1 (MQ=255) tACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGt < 1:158866/48‑1 (MQ=255) tACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGcaa < 1:673793/71‑3 (MQ=255) cTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCaa < 1:2602418/70‑1 (MQ=255) acaTTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAata > 1:2842451/1‑71 (MQ=255) acaTTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAata > 1:96629/1‑71 (MQ=255) acaTTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAat < 1:1605180/70‑1 (MQ=255) acaTTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAat > 1:92117/1‑70 (MQ=255) acaTTGACGACACGCAGGTTCACGGCAATCTGATCAAGCTGGTAt < 1:3135310/45‑1 (MQ=38) acaTTGACGACACGCAGGTTCACGGCAATCTGATCAAGCTGGTAt < 1:1249858/45‑1 (MQ=38) caTTGACGACACGCAGGTTCACGGCAATCTGATCAAGCTGGTAt < 1:1915196/44‑1 (MQ=38) ttGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGt < 1:399065/40‑1 (MQ=255) gacgacGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCtt < 1:3400981/70‑1 (MQ=255) gacgacGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCtt > 1:3187637/1‑70 (MQ=255) gacgacGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCTTg > 1:3439090/1‑71 (MQ=255) acgacgCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCtt < 1:2920769/69‑1 (MQ=255) cgacgCGCAGGTTCACGGCAATCTGAGCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCTTg < 1:1011729/69‑1 (MQ=255) acgCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCTTGcccc < 1:591714/71‑1 (MQ=255) gcgcAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCTTg > 1:521013/1‑65 (MQ=255) | TCATAGGAAAGTATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGGCACCGATGCCAAAATATCTTGCCCC > W3110S.gb/1101327‑1101463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |