| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,174,888 | C→A | 13.8% | P218P (CCG→CCT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,174,888 | 0 | C | A | 13.8% | 103.3 / 3.7 | 36 | P218P (CCG→CCT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base C (18/13); new base A (3/2); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
CGCTTCACTTCGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCA > W3110S.gb/1174826‑1174957 | cgcCTCACTTCGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTc < 1:2281843/71‑1 (MQ=255) gcTTCACTTCGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCg > 1:466090/1‑71 (MQ=255) cTTCACTTCGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGt > 1:2345125/1‑71 (MQ=255) cACTTCGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGaaa > 1:1828670/1‑63 (MQ=255) cACTTCGAAGGTATCACGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTAGGAAATTCGTgcg > 1:3760135/1‑71 (MQ=255) cGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCggg > 1:494257/1‑70 (MQ=255) aaGGTATCACGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTAGGAAATTCGt < 1:2961361/61‑1 (MQ=255) aaGGTATCACGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTAGGAAATTCGt < 1:860779/61‑1 (MQ=255) gTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGgcagca < 1:153341/70‑1 (MQ=255) tCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGAtt < 1:1844787/71‑1 (MQ=255) gcgcCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGa > 1:2774547/1‑71 (MQ=255) gcgcCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGa > 1:1813199/1‑71 (MQ=255) cTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGc > 1:253612/1‑69 (MQ=255) cTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGc > 1:658405/1‑69 (MQ=255) ggCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTc > 1:702314/1‑43 (MQ=255) ggCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTc > 1:156092/1‑43 (MQ=255) gatGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTc < 1:130557/67‑1 (MQ=255) gCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTc < 1:770083/69‑1 (MQ=255) gCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCt < 1:1128110/70‑1 (MQ=255) aCAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTtcctc > 1:3245665/1‑70 (MQ=255) gTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTg < 1:1088933/50‑1 (MQ=255) ttCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGgcagca < 1:662845/44‑1 (MQ=255) ttCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGcagcag < 1:3142819/45‑1 (MQ=255) cAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGt > 1:3252121/1‑70 (MQ=255) cAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTg > 1:969550/1‑71 (MQ=255) cAATTGCCGCTTTATCGGTAGGAAATTCGTGCGCGGGAAGCAGATTGATCTCTTCTACTTCCTCCAGCGTg > 1:511917/1‑71 (MQ=255) cAATTGCCGCTTTATCGGTAGGAAATTCGTGCGCGGGAAGCAGATTGATCTCTTCTACTTCCTCCAGCGTg > 1:130270/1‑71 (MQ=255) aaTTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTg < 1:2213151/70‑1 (MQ=255) ttGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGAt > 1:2309705/1‑46 (MQ=255) ttGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGAt > 1:2220202/1‑46 (MQ=255) tGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTtcctcc > 1:1594337/1‑61 (MQ=255) gCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTa > 1:259809/1‑52 (MQ=255) ccGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCgctg < 1:179395/70‑1 (MQ=255) tATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTc < 1:283131/70‑1 (MQ=255) tATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTc < 1:2656492/70‑1 (MQ=255) aTCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGa > 1:3413133/1‑71 (MQ=255) tcggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCa < 1:3412274/71‑1 (MQ=255) | CGCTTCACTTCGAAGGTATCGCGCCACTGGCTGCGGAACAGTTCAATTGCCGCTTTATCGGTCGGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCA > W3110S.gb/1174826‑1174957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |