Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,180,007 C→T 12.0% L371L (CTG→TTG)  lolE → outer membrane‑specific lipoprotein transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,180,0070CT12.0% 151.1 / 10.0 58L371L (CTG→TTG) lolEouter membrane‑specific lipoprotein transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (35/16);  new base T (2/5);  total (37/21)
Fisher's exact test for biased strand distribution p-value = 8.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGG  >  W3110S.gb/1179940‑1180076
                                                                   |                                                                     
tattGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGc                                                                    >  1:2614801/1‑71 (MQ=255)
tattGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGc                                                                    >  1:1399970/1‑71 (MQ=255)
 attGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGc                                                                    <  1:2549534/70‑1 (MQ=255)
 attGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGc                                                                    >  1:2120215/1‑70 (MQ=255)
        ggATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:1765300/1‑71 (MQ=255)
           ttGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGGGATATCTATTTTATTGATTTCCTGCCATCGGAATTg                                                         <  1:579339/71‑1 (MQ=255)
               aaaGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCAct                                                     >  1:2366105/1‑71 (MQ=255)
                   ttGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCgg                                                              <  1:1368625/58‑1 (MQ=255)
                     gATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTg                                                                     >  1:3315843/1‑49 (MQ=255)
                     gATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCActggctg                                                >  1:2112716/1‑70 (MQ=255)
                           tcatcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCAatggctgg                                               <  1:3766986/65‑1 (MQ=255)
                           tcatcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtct                                         >  1:1770283/1‑71 (MQ=255)
                           tcatcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtct                                         >  1:1685502/1‑71 (MQ=255)
                             atgaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       >  1:740760/4‑71 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCActggc                                                  >  1:2191472/1‑60 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       >  1:2823371/1‑71 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       >  1:2811975/1‑71 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       >  1:3745098/1‑71 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       <  1:1974488/71‑1 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       <  1:1983618/71‑1 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       <  1:3315942/71‑1 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       <  1:2397375/71‑1 (MQ=255)
                             atcaGTTCCTCTCCAGCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       <  1:2610725/71‑1 (MQ=255)
                               caGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:554669/1‑48 (MQ=255)
                               caGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:3830404/1‑48 (MQ=255)
                                aGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:601981/1‑47 (MQ=255)
                                aGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:319719/1‑47 (MQ=255)
                                aGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:3025475/1‑47 (MQ=255)
                                aGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCCGCCATCGGaa                                                            >  1:3746125/1‑47 (MQ=255)
                                 gTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACg                                   <  1:2515343/71‑1 (MQ=255)
                                 gTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACg                                   <  1:695462/71‑1 (MQ=255)
                                 gTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACg                                   <  1:3092063/71‑1 (MQ=255)
                                  ttCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGc                                                        <  1:829231/49‑1 (MQ=255)
                                    ccTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTAc                                >  1:2769624/1‑71 (MQ=255)
                                    ccTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTAc                                >  1:605346/1‑71 (MQ=255)
                                    ccTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTAc                                >  1:1285839/1‑71 (MQ=255)
                                        tcCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGt                            >  1:2536646/1‑71 (MQ=255)
                                        tcCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGt                            >  1:1508160/1‑71 (MQ=255)
                                        tcCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGt                            >  1:1797312/1‑71 (MQ=255)
                                        tcCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCATCTACGTACTGGt                            >  1:3757653/1‑71 (MQ=255)
                                          cAGCGATATCTATTTTATTGATTTCCTGCCATCGGaa                                                            >  1:2106581/1‑37 (MQ=255)
                                          cAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTa                                 >  1:1722639/1‑64 (MQ=255)
                                           aGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTcac                         <  1:345063/71‑1 (MQ=255)
                                           aGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTcac                         <  1:936204/71‑1 (MQ=255)
                                            gCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCa                                                       >  1:2589978/1‑40 (MQ=37)
                                            gCGATATCTATTTTATTGACTTCTTGCCATCGGAATTGCa                                                       >  1:608689/1‑40 (MQ=37)
                                             cGATATCTATTTTATTGATTTCCTGCCATAGGAATTGCACTGGCTGGACGTCTTCTaag                                   <  1:186727/59‑3 (MQ=255)
                                                tatCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCAt                    <  1:1258762/71‑1 (MQ=255)
                                                  tCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTAc                                    >  1:481371/1‑53 (MQ=255)
                                                  tCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACg                                   <  1:3061476/54‑1 (MQ=255)
                                                  tCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACg                                   <  1:2205723/54‑1 (MQ=255)
                                                           ttGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtcttc                                       <  1:1295989/41‑1 (MQ=255)
                                                            tGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCa                     >  1:3122599/1‑58 (MQ=255)
                                                            tGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCa                     >  1:2373375/1‑58 (MQ=255)
                                                              aTTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCt       <  1:940211/70‑1 (MQ=255)
                                                               tttCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCttt     >  1:2139018/1‑71 (MQ=255)
                                                                 tCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCtttt    >  1:3750403/1‑70 (MQ=255)
                                                                 tCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTg   >  1:215773/1‑71 (MQ=255)
                                                                  ccTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTgg  >  1:2303785/1‑71 (MQ=255)
                                                                  ccTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTgg  >  1:1108760/1‑71 (MQ=255)
                                                                   |                                                                     
TATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGG  >  W3110S.gb/1179940‑1180076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: