| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,237,886 | A→G | 11.6% | F311F (TTT→TTC) | ycgB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,237,886 | 0 | A | G | 11.6% | 105.2 / 5.9 | 43 | F311F (TTT→TTC) | ycgB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (29/9); new base G (2/3); total (31/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAG > W3110S.gb/1237817‑1237955 | cGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCaaa > 1:1060821/1‑70 (MQ=255)cGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCaaa > 1:13135/1‑70 (MQ=255)cGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCaaa > 1:2502818/1‑70 (MQ=255)cGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCaaa > 1:2306334/1‑70 (MQ=255) gATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACt > 1:3551733/1‑68 (MQ=255) gATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTc > 1:681932/1‑69 (MQ=255) gCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCa > 1:3100027/1‑71 (MQ=255) gCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATaa < 1:3337030/71‑1 (MQ=255) gTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAAc > 1:390776/1‑71 (MQ=255) ccACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGtt > 1:3754598/1‑71 (MQ=255) ccACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGtt > 1:227359/1‑71 (MQ=255) cACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTc < 1:2256751/71‑1 (MQ=255) ttATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATaa < 1:3472971/55‑1 (MQ=255) tataGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTc > 1:3544795/1‑71 (MQ=255) ataGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAAc > 1:1989752/1‑43 (MQ=255) ataGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAAc > 1:1752664/1‑43 (MQ=255) ataGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAAc > 1:3678460/1‑43 (MQ=255) ataGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTcc > 1:2572133/1‑71 (MQ=255) gAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATa > 1:516255/1‑69 (MQ=255) gAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATa > 1:2174682/1‑69 (MQ=255) gAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCAGTTACTTTCCCTTCATCATa > 1:2855340/1‑69 (MQ=255) aaGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTAATTTCCCTTCATCATa > 1:1367076/1‑68 (MQ=255) gACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTAc > 1:2564221/1‑51 (MQ=255) gACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTAc > 1:590220/1‑51 (MQ=255) gACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTAc > 1:1568882/1‑51 (MQ=255) acaTTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGt > 1:2590270/1‑70 (MQ=255) caTTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt > 1:547359/1‑70 (MQ=255) caTTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACCTTCCGTTACTTTCCCTTCATCATACAGATGGt > 1:3816521/1‑69 (MQ=255) aTTGGTGTGGCTGTGCAAGAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtca < 1:2626244/55‑1 (MQ=255) aTTGGTGTGGCTGTGCAAGAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtca < 1:283686/55‑1 (MQ=255) aTTGGTGTGGCTGTGCAAGAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtca < 1:647906/55‑1 (MQ=255) ttGGTGTGGCTGTGCAAAAACTCCAGCATAGAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAg > 1:2524551/1‑71 (MQ=255) ttGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACttt < 1:3486200/47‑1 (MQ=255) ttGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACttt < 1:2742209/47‑1 (MQ=255) ttGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAg > 1:1995482/1‑71 (MQ=255) ttGGTGTGGATGTGCAAAAACTCCAGCATAAAACGTTCCGTTACttt < 1:2592819/47‑1 (MQ=255) tGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTc > 1:210068/1‑37 (MQ=255) ggTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTa > 1:1791490/1‑41 (MQ=255) ggTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTa > 1:127973/1‑41 (MQ=255) tgGCTGTGCAAGAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATgg > 1:350449/1‑60 (MQ=255) tgGCTGTGCAAGAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGgt > 1:2968836/1‑71 (MQ=255) gCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa > 1:1190584/1‑65 (MQ=255) cTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGgtg < 1:992692/69‑1 (MQ=255) tgCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtc < 1:2771176/41‑1 (MQ=255) cAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtcatca < 1:634369/43‑1 (MQ=255) cAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAg > 1:1849508/1‑67 (MQ=255) cAAAAACTCCAGCATAAAACGTTCCGTTAATTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAg > 1:820353/1‑67 (MQ=255) aaaaCTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAg < 1:3122182/71‑1 (MQ=255) aaaaCTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAg < 1:1395039/71‑1 (MQ=255) | CGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAG > W3110S.gb/1237817‑1237955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |