| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,285,540 | G→A | 43.7% | S120S (TCG→TCA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,285,540 | 0 | G | A | 43.7% | 1.3 / 14.5 | 16 | S120S (TCG→TCA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/3); new base A (4/3); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGACGATTATTACGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCGCAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGATGGCGAAAATCGAAAAAGGGCC > W3110S.gb/1285470‑1285605 | tCGACGATTATTACGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCg > 1:2202960/1‑71 (MQ=255) attattACGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCGCAGCCg < 1:1381371/71‑1 (MQ=255) taCGAGCCGTTCGATTTTGATTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCACAGCCGATTGc > 1:730696/1‑71 (MQ=255) taCGAGCCGTTCGATTTTGATTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCACAGCCGATTGc > 1:3354106/1‑71 (MQ=255) taCGAGCCGTTCGATTTTGATTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCACAGCCGATTGc > 1:2859320/1‑71 (MQ=255) taCGAGCCGTTCGATTTTGATTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCACAGCCGATTGc > 1:2348953/1‑71 (MQ=255) taCGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCGCAGCCGATTGc > 1:1371823/1‑71 (MQ=255) aCGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCGCAGCCGATTGcc > 1:1332936/1‑71 (MQ=255) tttGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCGCAGCCGATTGCCCGTCCGCGTTCGCt > 1:1188258/1‑71 (MQ=255) cATACCGCGCCGGAAGGCAGCAAATCGCAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGAt > 1:2083865/1‑71 (MQ=255) cgcgCCGGAAGGCAGCAAATCGCAGCCGATTGCCCGTCCGCGTTCGCTg > 1:19861/1‑49 (MQ=255) gcCGGAAGGCAGCAAATCGCAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGATGGCGaaaa < 1:1623103/71‑1 (MQ=255) ggagcaAATCACAGCCGATTGCCCGTCCGCGTTCACTGATTACCGGCGAACGGAt < 1:3722613/53‑1 (MQ=37) gcagcaAATCACAGCCGATTGCCCGTCCGCGTTCACTGATTACCGGCGAACGGAt < 1:2101052/55‑1 (MQ=37) gcagcaAATCACAGCCGATTGCCCGTCCGCGTTCACTGATTACCGGCGAACGGAt < 1:2669818/55‑1 (MQ=37) cagcaAATCGCAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGATGGCGAAAATCGAAAAAg < 1:1132446/71‑1 (MQ=255) aaTCGCAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGATGGCGAAAATCGAAAAAGGGcc > 1:219576/1‑70 (MQ=255) | TCGACGATTATTACGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCGCAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGATGGCGAAAATCGAAAAAGGGCC > W3110S.gb/1285470‑1285605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |