| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,302,548 | A→G | 13.1% | intergenic (+190/‑348) | insD → / → oppA | IS2 element protein/oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,302,548 | 0 | A | G | 13.1% | 90.8 / 6.2 | 38 | intergenic (+190/‑348) | insD/oppA | IS2 element protein/oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/19); new base G (3/2); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCA > W3110S.gb/1302487‑1302611 | aTCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCaa > 1:190793/1‑71 (MQ=255) ctctATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAt > 1:2501959/1‑70 (MQ=255) tctATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa > 1:2613006/1‑70 (MQ=255) tATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATaa < 1:1328362/69‑1 (MQ=255) tttGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATaaaa < 1:2154330/69‑1 (MQ=255) ttgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATaaaa > 1:3668881/1‑68 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGTATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:1976648/70‑1 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:1980934/70‑1 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:764658/70‑1 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:860016/70‑1 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag > 1:3246062/1‑70 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:147905/70‑1 (MQ=255) tgattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:3070926/70‑1 (MQ=255) gattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATaaaa < 1:3354167/66‑1 (MQ=255) gattgaATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:1117715/69‑1 (MQ=255) gaaAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTaa > 1:3666732/1‑70 (MQ=255) aaaGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAAc < 1:2043366/70‑1 (MQ=255) tgttAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGc > 1:3209703/1‑69 (MQ=255) gttAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCaa < 1:172067/70‑1 (MQ=255) gttAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCaa < 1:383562/70‑1 (MQ=255) aGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGt < 1:3693819/71‑1 (MQ=255) gCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTa < 1:887750/70‑1 (MQ=255) gCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTAt > 1:2611926/1‑71 (MQ=255) gATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGc > 1:3400012/1‑70 (MQ=255) tGCAAGCTATAGATTCTGATACGGTCAATAAAagag < 1:2824530/36‑1 (MQ=255) aGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAg < 1:2492140/71‑1 (MQ=255) cTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGaa < 1:1502665/71‑1 (MQ=255) aGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAAcc > 1:694090/1‑69 (MQ=255) gATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCg < 1:2511172/69‑1 (MQ=255) gATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCa > 1:508539/1‑71 (MQ=255) aTTCTGGTTCGGTCAATAAAAGATAATTGCTTAACAATTTTGCAAAATGt < 1:3515451/50‑1 (MQ=255) aTTCTGGTTCGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGt < 1:2152024/50‑1 (MQ=255) ttCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCa > 1:3498833/1‑69 (MQ=255) tCTGGTTCGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAAt > 1:2145255/1‑46 (MQ=39) tCTGGTTCGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAAt > 1:639747/1‑46 (MQ=39) tCTGGTTCGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAAt > 1:66697/1‑46 (MQ=39) cTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTAtt > 1:832086/1‑50 (MQ=255) cTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTAtt > 1:3398925/1‑50 (MQ=255) aTACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCaaaa > 1:570393/1‑41 (MQ=255) | ATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCA > W3110S.gb/1302487‑1302611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |