Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 126.7 / NA 45Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/21);  total (24/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303567‑1303695
                                                                |                                                                
aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                            >  1:3191839/1‑71 (MQ=255)
   cgaaggaaTCGTTCTTGAAGGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                          >  1:3634912/1‑70 (MQ=255)
    gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:1466416/71‑1 (MQ=255)
       cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGAGAAAACCGTTATTAACTATGTAACCTAtt                                                      >  1:388038/1‑70 (MQ=255)
             gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                               >  1:3115621/1‑71 (MQ=255)
             gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                               >  1:3111249/1‑71 (MQ=255)
              ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                          <  1:878735/59‑1 (MQ=255)
               tCTTGAACGCAGCCCGCCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                              >  1:3434323/1‑70 (MQ=255)
                 ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                           >  1:1822560/1‑71 (MQ=255)
                     aCGCAGCCCGACCTACTTGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       <  1:1526591/71‑1 (MQ=255)
                       gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                        >  1:1256082/1‑68 (MQ=255)
                       gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       >  1:702128/1‑69 (MQ=255)
                        cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                    <  1:1850513/71‑1 (MQ=255)
                           cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                 >  1:3116236/1‑71 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               >  1:9542/1‑71 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               >  1:584801/1‑71 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               <  1:3850598/71‑1 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               <  1:1644997/71‑1 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               >  1:2958765/1‑71 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                                >  1:622310/1‑70 (MQ=255)
                             cGACATACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                                >  1:598459/1‑70 (MQ=255)
                                 cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                            <  1:1827351/70‑1 (MQ=255)
                                  tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:3233466/41‑1 (MQ=38)
                                  tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:1580459/41‑1 (MQ=38)
                                  tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:39446/41‑1 (MQ=38)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                        <  1:1330267/71‑1 (MQ=255)
                                     tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                              <  1:550207/64‑1 (MQ=255)
                                     tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa                       >  1:2544279/1‑71 (MQ=255)
                                      ggAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                       >  1:1024959/1‑38 (MQ=37)
                                       gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         >  1:3666995/1‑51 (MQ=255)
                                       gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     >  1:3326468/1‑55 (MQ=255)
                                         acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGc                   <  1:2928469/71‑1 (MQ=255)
                                              gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa                       >  1:3796272/1‑62 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt             <  1:397729/71‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt             <  1:901489/71‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGACTATTGCTTCTGAAGTTAACGATGTCAACCGCTACCGt             <  1:2398658/71‑1 (MQ=255)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               <  1:33854/52‑1 (MQ=255)
                                                gAAAACCGCTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa            <  1:2334538/71‑1 (MQ=255)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                    >  1:2084385/1‑45 (MQ=38)
                                                       gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGa     <  1:2297870/71‑1 (MQ=255)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     >  1:979939/1‑36 (MQ=37)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt  >  1:668184/1‑71 (MQ=255)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt  >  1:3154936/1‑71 (MQ=255)
                                                           ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt                  <  1:613050/54‑1 (MQ=255)
                                                           ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt                  <  1:274524/54‑1 (MQ=255)
                                                                |                                                                
AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303567‑1303695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: