| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 129.5 / NA | 45 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (24/21); total (24/21) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT > W3110S.gb/1303567‑1303695 | aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa > 1:3191839/1‑71 (MQ=255) cgaaggaaTCGTTCTTGAAGGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc > 1:3634912/1‑70 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1466416/71‑1 (MQ=255) cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGAGAAAACCGTTATTAACTATGTAACCTAtt > 1:388038/1‑70 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:3115621/1‑71 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:3111249/1‑71 (MQ=255) ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:878735/59‑1 (MQ=255) tCTTGAACGCAGCCCGCCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt > 1:3434323/1‑70 (MQ=255) ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt > 1:1822560/1‑71 (MQ=255) aCGCAGCCCGACCTACTTGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:1526591/71‑1 (MQ=255) gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt > 1:1256082/1‑68 (MQ=255) gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg > 1:702128/1‑69 (MQ=255) cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg < 1:1850513/71‑1 (MQ=255) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa > 1:3116236/1‑71 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc > 1:9542/1‑71 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc > 1:584801/1‑71 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:3850598/71‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1644997/71‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc > 1:2958765/1‑71 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc > 1:622310/1‑70 (MQ=255) cGACATACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc > 1:598459/1‑70 (MQ=255) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt < 1:1827351/70‑1 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:3233466/41‑1 (MQ=38) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1580459/41‑1 (MQ=38) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:39446/41‑1 (MQ=38) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa < 1:1330267/71‑1 (MQ=255) tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg < 1:550207/64‑1 (MQ=255) tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa > 1:2544279/1‑71 (MQ=255) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTAt > 1:1024959/1‑38 (MQ=37) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc > 1:3666995/1‑51 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa > 1:3326468/1‑55 (MQ=255) acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGc < 1:2928469/71‑1 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa > 1:3796272/1‑62 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:397729/71‑1 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:901489/71‑1 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGACTATTGCTTCTGAAGTTAACGATGTCAACCGCTACCGt < 1:2398658/71‑1 (MQ=255) gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:33854/52‑1 (MQ=255) gAAAACCGCTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa < 1:2334538/71‑1 (MQ=255) aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:2084385/1‑45 (MQ=38) gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGa < 1:2297870/71‑1 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGaa > 1:979939/1‑36 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:668184/1‑71 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:3154936/1‑71 (MQ=255) ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt < 1:613050/54‑1 (MQ=255) ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt < 1:274524/54‑1 (MQ=255) | AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT > W3110S.gb/1303567‑1303695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |