| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,313,544 | A→G | 42.8% | intergenic (+22/+18) | tonB → / ← yciA | membrane spanning protein in TonB‑ExbB‑ExbD complex/predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,313,544 | 0 | A | G | 42.8% | 2.4 / 29.3 | 29 | intergenic (+22/+18) | tonB/yciA | membrane spanning protein in TonB‑ExbB‑ExbD complex/predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (5/11); new base G (7/5); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01 | |||||||||||
GTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTC > W3110S.gb/1313475‑1313594 | gtggtgAATATCCTGTTTAAAATTAACGTCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAc > 1:2172711/1‑71 (MQ=255)gtggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAc > 1:2388109/1‑71 (MQ=255) gAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAg > 1:2365984/1‑71 (MQ=255) tatCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCt < 1:3599729/71‑1 (MQ=255) tatCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGGCAAAAGCCTCCGACCGGAGGCt < 1:1312857/71‑1 (MQ=255) tttAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCtttt < 1:92652/67‑1 (MQ=255) tttAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACt < 1:592235/71‑1 (MQ=255) aaaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTAt < 1:3733294/70‑1 (MQ=255) aaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAcc < 1:1753052/52‑1 (MQ=255) tAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGCCCGGAGGCttt > 1:3838885/1‑58 (MQ=255) aCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAcc < 1:71880/47‑1 (MQ=255) caccacCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGt < 1:699770/71‑1 (MQ=255) ttaattaacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGAGGCtttt < 1:2514529‑M2/29‑1 (MQ=255) accaccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGt < 1:397665/70‑1 (MQ=255) taattaacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGa > 1:1351664‑M2/23‑53 (MQ=255) aattaacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGActaca > 1:847068‑M2/22‑55 (MQ=255) attaacggcactcctcagccAAGTCAAAAGCCTCCGGGCGGAGGCttt < 1:3574222‑M2/28‑1 (MQ=255) ttaacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGa > 1:1586356‑M2/20‑41 (MQ=255) ccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGgc > 1:2247100/1‑71 (MQ=255) gAAATTCAGTAAGCAGAAAGTC‑AAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGgcgc > 1:1941081/1‑70 (MQ=255) aacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGa > 1:3681104‑M2/18‑39 (MQ=255) aacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGa > 1:1675932‑M2/18‑39 (MQ=255) aacggcactcctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGa > 1:586721‑M2/18‑48 (MQ=255) actcctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTa < 1:3440577‑M2/34‑1 (MQ=255) ctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGActaca > 1:745026‑M2/8‑41 (MQ=255) ctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTa < 1:3661786‑M2/34‑1 (MQ=255) ctcagccAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTa < 1:3554717‑M2/34‑1 (MQ=255) cAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTc < 1:1031040/71‑1 (MQ=255) aaaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGttt < 1:691256/56‑1 (MQ=255) | GTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTC > W3110S.gb/1313475‑1313594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |