| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,355,756 | G→A | 8.9% | S159S (TCC→TCT) | sapC ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,355,756 | 0 | G | A | 8.9% | 126.0 / 2.6 | 45 | S159S (TCC→TCT) | sapC | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (16/25); new base A (2/2); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
ATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTAT > W3110S.gb/1355687‑1355826 | aTGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTgg < 1:864607/70‑1 (MQ=255) cACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACa < 1:3314402/71‑1 (MQ=255) aCCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACaa < 1:2256321/71‑1 (MQ=255) aCCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACa < 1:1044130/70‑1 (MQ=255) aCCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACa < 1:711415/70‑1 (MQ=255) aTGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATGGCGTgaga > 1:1930072/1‑65 (MQ=255) aTGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATGGCGTGAGACAAACt > 1:777629/1‑71 (MQ=255) aTGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACt < 1:923855/71‑1 (MQ=255) aTGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACt < 1:3126739/71‑1 (MQ=255) cTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCgg > 1:2904114/1‑71 (MQ=255) tAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTc < 1:1498566/66‑1 (MQ=255) aaTCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCgg > 1:841247/1‑71 (MQ=255) aaTCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCgg > 1:475263/1‑71 (MQ=255) aTCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGc < 1:170402/71‑1 (MQ=255) tCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGc > 1:3160975/1‑70 (MQ=255) gAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATGGCGTGAGACAAACTc < 1:1163952/60‑1 (MQ=255) gAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATGGCGTGAGACAAACTc < 1:1630742/60‑1 (MQ=255) gAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCaaa > 1:1302168/1‑71 (MQ=255) tACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTc < 1:1835138/50‑1 (MQ=255) tgcgGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCgg > 1:2933303/2‑50 (MQ=255) cgcgGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAAc > 1:2988024/1‑46 (MQ=255) cgcgGCAGCAATGCCAGCCAGACAGAAAACATAGCGTGGGACAAACTCGGTCCGGCaaa > 1:207468/1‑59 (MQ=255) gcgGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATa < 1:668995/71‑1 (MQ=255) gcgGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATa < 1:3477222/71‑1 (MQ=255) ggCAGCAATGCCAGCCAGACAGCAAACATGGCGTGAGACAAACTCGGTCCGGCAAACGCTACaa > 1:2891542/1‑64 (MQ=255) gcagcaATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACt < 1:1362574/43‑1 (MQ=255) gcagcaATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACt < 1:3471013/43‑1 (MQ=255) gcagcaATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAAtt < 1:3756111/71‑1 (MQ=255) agcaATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCg > 1:1326411/1‑43 (MQ=255) agcaATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATaa > 1:2826319/1‑67 (MQ=255) caATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCa > 1:2524217/1‑49 (MQ=255) tGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCaa > 1:2004506/1‑69 (MQ=255) gccagccaGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTAc < 1:661838/53‑1 (MQ=255) ccagccagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAAcagc < 1:3678112/71‑1 (MQ=255) ccagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCg < 1:1552356/71‑1 (MQ=255) cagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAAca > 1:3181833/1‑64 (MQ=255) cagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAAca > 1:1747729/1‑64 (MQ=255) cagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCg < 1:1556865/70‑1 (MQ=255) agACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGaa < 1:1607551/71‑1 (MQ=255) cAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAgg > 1:1500965/1‑70 (MQ=255) cAGCAAACATAGCGTGGGACAAACTCGGTACGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAggg < 1:2859245/71‑1 (MQ=255) gCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGa < 1:21265/70‑1 (MQ=255) gCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGAt > 1:866718/1‑71 (MQ=255) aaCTTAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGaa < 1:1663271/63‑1 (MQ=255) aaCATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGaa < 1:75083/63‑1 (MQ=255) aaCATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAACGGATCGc < 1:1739556/71‑1 (MQ=255) aTAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCg < 1:2815347/58‑1 (MQ=255) ggACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTAt > 1:3820197/1‑71 (MQ=255) | ATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTAT > W3110S.gb/1355687‑1355826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |