| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,442,292 | G→A | 8.7% | G69G (GGC→GGT) | ydbK ← | fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,442,292 | 0 | G | A | 8.7% | 131.2 / 2.0 | 46 | G69G (GGC→GGT) | ydbK | fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (28/14); new base A (2/2); total (30/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAA > W3110S.gb/1442223‑1442359 | cGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAgcgc < 1:1684064/71‑1 (MQ=255)cGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAgcgc < 1:2469863/71‑1 (MQ=255) tCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCa > 1:965905/1‑71 (MQ=255) tCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCa < 1:994152/71‑1 (MQ=255) ggATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATg > 1:3287086/1‑70 (MQ=255) gATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCa > 1:2144974/1‑71 (MQ=255) tcatcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCAt > 1:3701839/1‑66 (MQ=255) tcatcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACg > 1:1872921/1‑71 (MQ=255) catcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACgg > 1:1869405/1‑71 (MQ=255) catcaGAAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACgg > 1:9125/1‑71 (MQ=255) gcagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCg < 1:2114132/71‑1 (MQ=255) cagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGa < 1:1162788/71‑1 (MQ=255) aaaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAg > 1:3444443/1‑70 (MQ=255) aaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGc < 1:559067/64‑1 (MQ=255) aaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGc < 1:43439/64‑1 (MQ=255) aaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGc < 1:2955729/64‑1 (MQ=255) aaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGc < 1:2864628/64‑1 (MQ=255) cTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAg < 1:187065/65‑1 (MQ=255) tGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCAccc < 1:507963/69‑1 (MQ=255) tGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCAccc < 1:2824928/69‑1 (MQ=255) cGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAg > 1:413732/1‑62 (MQ=255) cGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAg > 1:1448481/1‑62 (MQ=255) aTGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAgcgc < 1:2366216/42‑1 (MQ=255) aTGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAgcgc < 1:976418/42‑1 (MQ=255) gACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGa > 1:213019/1‑71 (MQ=255) aaCGATGTTGAAAGTTCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCAt > 1:3252903/1‑71 (MQ=255) aaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGa > 1:2901871/1‑49 (MQ=255) aaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGa > 1:2973211/1‑49 (MQ=255) aaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGa > 1:1324644/1‑49 (MQ=255) aaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCAt > 1:2515436/1‑71 (MQ=255) ttGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAAc < 1:1012889/70‑1 (MQ=255) gAAAGGTCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc > 1:249/1‑71 (MQ=255) gAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCa > 1:460353/1‑70 (MQ=255) gAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc > 1:3283919/1‑71 (MQ=255) aaGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgc < 1:681553/71‑1 (MQ=255) ggCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgtg > 1:1763711/1‑71 (MQ=255) cACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgtggt < 1:3481199/71‑1 (MQ=255) aCCAGTTTGCAAAGCACCATGCACGGTAGCGATAGCACCCGTTTCCGACTGCATTTc > 1:1013577/1‑57 (MQ=255) aCCAGTTTGCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCAttt > 1:3105987/1‑56 (MQ=255) cGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgtgt < 1:663336/71‑1 (MQ=255) gtttGCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtc < 1:1555577/68‑1 (MQ=255) gtttGCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtc < 1:1057982/68‑1 (MQ=255) gTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcg > 1:3764458/1‑60 (MQ=255) tGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAGGCGTGGTGTGTCTcc > 1:3203817/1‑71 (MQ=255) aaGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCa > 1:3639828/1‑70 (MQ=255) aaGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCa > 1:471452/1‑70 (MQ=255) aaGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCa > 1:2513879/1‑70 (MQ=255) aGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCaaa > 1:400580/1‑71 (MQ=255) aGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCaaa > 1:2416204/1‑71 (MQ=255) aGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCaaa > 1:1403379/1‑71 (MQ=255) | CGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAA > W3110S.gb/1442223‑1442359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |