Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,662,156 C→G 20.3% L792V (CTG→GTG)  ynfE → oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,662,1560CG20.3% 49.7 / 6.6 27L792V (CTG→GTG) ynfEoxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base C (8/13);  new base G (3/2);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAC  >  W3110S.gb/1662086‑1662220
                                                                      |                                                                
cAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcaccac                                                                  <  1:2005038/71‑1 (MQ=255)
    atGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGc                                                              >  1:2365330/1‑71 (MQ=255)
     tGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCg                                                             <  1:1255169/71‑1 (MQ=255)
         tGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGaa                                                           <  1:2566272/69‑1 (MQ=255)
             gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                     <  1:1506212/71‑1 (MQ=255)
             gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                     <  1:2049643/71‑1 (MQ=255)
                  aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgc                                                <  1:1461039/71‑1 (MQ=255)
                                      tgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGa                                                       <  1:2760375/44‑1 (MQ=255)
                                      tgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag                            <  1:920798/71‑1 (MQ=255)
                                        aaTACGCTAACCACTCTGCGCCCTTCACCATTGGCGAAGTGAAACCCGCAGCACACTAATCTGGTCgaga                           <  1:1466208/70‑1 (MQ=255)
                                          tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                     >  1:3775714/1‑42 (MQ=255)
                                          tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                     >  1:372104/1‑42 (MQ=255)
                                                 aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                  <  1:452962/70‑1 (MQ=255)
                                                 aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                  <  1:3781017/70‑1 (MQ=255)
                                                 aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa                   <  1:540434/69‑1 (MQ=255)
                                                     ctctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATaa             <  1:2660565/71‑1 (MQ=255)
                                                     ctctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATaa             <  1:2787579/71‑1 (MQ=255)
                                                        tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc           >  1:2235892/1‑70 (MQ=255)
                                                        tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc           >  1:2162074/1‑70 (MQ=255)
                                                          cgcCCTTCACCAGTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc           <  1:505741/68‑1 (MQ=255)
                                                          cgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc        >  1:1682495/1‑71 (MQ=255)
                                                             ccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg                              <  1:2381800/46‑1 (MQ=255)
                                                             ccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGa      >  1:1494948/1‑70 (MQ=255)
                                                               ttCGCCAGTGGTGAAGGGCAACCCGCAGCACACTAATCTGGTCga                             >  1:1486952/1‑45 (MQ=21)
                                                               ttCGCCAGTGGTGAAGGGCAACCCGCAGCACACTAATCTGGTCga                             >  1:2599664/1‑45 (MQ=21)
                                                               ttCGCCAGTGGTGAAGGGCAACCCGCAGCACACTAATCTGGTCga                             >  1:760113/1‑45 (MQ=21)
                                                               ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCga                             >  1:2616416/1‑45 (MQ=255)
                                                               ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                  <  1:652115/56‑1 (MQ=255)
                                                                 caccacTGGCGAAGGGAAACCCGCAGCACACTAAt                                     >  1:3134667/1‑35 (MQ=255)
                                                                 caccacTGGCGAAGGGAAACCCGCAGCACACTAAt                                     >  1:849300/1‑35 (MQ=255)
                                                                 caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAata                >  1:504979/1‑56 (MQ=255)
                                                                 caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGAc     >  1:1117499/1‑67 (MQ=255)
                                                                 caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc  >  1:3649459/1‑70 (MQ=255)
                                                                 caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc  >  1:1985120/1‑70 (MQ=255)
                                                                  accacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCg                        >  1:2041071/1‑47 (MQ=255)
                                                                      |                                                                
CAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAC  >  W3110S.gb/1662086‑1662220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: