Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,662,156 | C→G | 20.3% | L792V (CTG→GTG) | ynfE → | oxidoreductase subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,662,156 | 0 | C | G | 20.3% | 49.7 / 6.6 | 27 | L792V (CTG→GTG) | ynfE | oxidoreductase subunit |
Reads supporting (aligned to +/- strand): ref base C (8/13); new base G (3/2); total (11/16) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.20e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 |
CAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAC > W3110S.gb/1662086‑1662220 | cAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcaccac < 1:2005038/71‑1 (MQ=255) atGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGc > 1:2365330/1‑71 (MQ=255) tGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCg < 1:1255169/71‑1 (MQ=255) tGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGaa < 1:2566272/69‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa < 1:1506212/71‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa < 1:2049643/71‑1 (MQ=255) aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgc < 1:1461039/71‑1 (MQ=255) tgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGa < 1:2760375/44‑1 (MQ=255) tgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag < 1:920798/71‑1 (MQ=255) aaTACGCTAACCACTCTGCGCCCTTCACCATTGGCGAAGTGAAACCCGCAGCACACTAATCTGGTCgaga < 1:1466208/70‑1 (MQ=255) tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa > 1:3775714/1‑42 (MQ=255) tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa > 1:372104/1‑42 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa < 1:452962/70‑1 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa < 1:3781017/70‑1 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa < 1:540434/69‑1 (MQ=255) ctctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATaa < 1:2660565/71‑1 (MQ=255) ctctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATaa < 1:2787579/71‑1 (MQ=255) tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 1:2235892/1‑70 (MQ=255) tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 1:2162074/1‑70 (MQ=255) cgcCCTTCACCAGTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc < 1:505741/68‑1 (MQ=255) cgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc > 1:1682495/1‑71 (MQ=255) ccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg < 1:2381800/46‑1 (MQ=255) ccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGa > 1:1494948/1‑70 (MQ=255) ttCGCCAGTGGTGAAGGGCAACCCGCAGCACACTAATCTGGTCga > 1:1486952/1‑45 (MQ=21) ttCGCCAGTGGTGAAGGGCAACCCGCAGCACACTAATCTGGTCga > 1:2599664/1‑45 (MQ=21) ttCGCCAGTGGTGAAGGGCAACCCGCAGCACACTAATCTGGTCga > 1:760113/1‑45 (MQ=21) ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCga > 1:2616416/1‑45 (MQ=255) ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa < 1:652115/56‑1 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:3134667/1‑35 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:849300/1‑35 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAata > 1:504979/1‑56 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGAc > 1:1117499/1‑67 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc > 1:3649459/1‑70 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc > 1:1985120/1‑70 (MQ=255) accacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCg > 1:2041071/1‑47 (MQ=255) | CAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAC > W3110S.gb/1662086‑1662220 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |