| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,724,458 | A→T | 7.9% | A208A (GCA→GCT) | ydhK → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,724,458 | 0 | A | T | 7.9% | 163.9 / 6.5 | 63 | A208A (GCA→GCT) | ydhK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (29/29); new base T (2/3); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GCACGCCCGATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGC > W3110S.gb/1724392‑1724524 | gCACGCCCGATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTgcagca > 1:2341713/1‑70 (MQ=255) cGCCCGATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCATCACATg < 1:497522/71‑1 (MQ=255) cGCCCGATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATg < 1:665926/71‑1 (MQ=255) cGCCCGATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATg < 1:670569/71‑1 (MQ=255) gATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAgg < 1:3397436/70‑1 (MQ=255) aTTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAgggg > 1:3174601/1‑71 (MQ=255) ttACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGt > 1:2918979/1‑71 (MQ=255) ttACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGt < 1:274971/71‑1 (MQ=255) aCTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTg < 1:2923578/70‑1 (MQ=255) aCTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTg < 1:3436686/70‑1 (MQ=255) aCTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTg < 1:742036/70‑1 (MQ=255) tGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTgca < 1:1567113/53‑1 (MQ=255) tGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTgca < 1:3582177/53‑1 (MQ=255) gAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGcac > 1:188990/1‑55 (MQ=255) gAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGcac > 1:1907195/1‑55 (MQ=255) gAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTg < 1:2832543/70‑1 (MQ=255) gAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTg < 1:3551435/70‑1 (MQ=255) aaCATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTggg < 1:649320/71‑1 (MQ=255) aCATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTggg > 1:3760219/1‑70 (MQ=255) cATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCa < 1:2705908/71‑1 (MQ=255) aTGCCAGTTTACTCTGGCAGCCTGAAACAAACGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAg < 1:3782165/71‑1 (MQ=255) tGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATg < 1:3694021/53‑1 (MQ=255) tGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGa < 1:513156/71‑1 (MQ=255) gCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTggg > 1:2289261/1‑66 (MQ=255) cAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATAc > 1:2001539/1‑71 (MQ=255) gTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTg < 1:3856674/56‑1 (MQ=255) tttACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGcaca < 1:1047637/45‑1 (MQ=255) tttACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGa < 1:1341348/48‑1 (MQ=255) tttACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGt < 1:3465269/54‑1 (MQ=255) ttACTCTGGCAGCCTGAAACAACCGATGCCATTCGTgcagca < 1:640877/42‑1 (MQ=255) ttACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGa > 1:3563526/1‑64 (MQ=255) ttACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGAc > 1:2924164/1‑71 (MQ=255) tACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGAc > 1:3325386/1‑70 (MQ=255) tACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGAc > 1:1474708/1‑70 (MQ=255) tACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGAc > 1:1134732/1‑70 (MQ=255) tACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGAc > 1:110728/1‑70 (MQ=255) tGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGa > 1:2715808/1‑49 (MQ=255) tGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGaa > 1:619112/1‑71 (MQ=255) ggCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAAt > 1:2598024/1‑71 (MQ=255) gCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAAtt < 1:2996230/71‑1 (MQ=255) gCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGA‑CATGAAtt < 1:3007440/70‑1 (MQ=255) cAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAAttt < 1:3541113/71‑1 (MQ=255) ccTGAAACAACCGATGCCATTCGTGCAGCACATGaa < 1:820747/36‑1 (MQ=255) ccTGAAACAACCGATGCCATTCGTGCAGCACATGaa < 1:501086/36‑1 (MQ=255) aaaCAACCGATGCCATTCGTGCAGCACATGAAggg > 1:1119925/1‑35 (MQ=255) aaaCAACCGATGCCATTCGTGCAGCACATGAAggg > 1:2442831/1‑35 (MQ=255) aaaCAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGa > 1:1794232/1‑48 (MQ=255) aaaCAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGa > 1:1877012/1‑48 (MQ=255) aaaCAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGa > 1:2121420/1‑48 (MQ=255) acaacCGTGGCAGTTCGTGCTGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGt > 1:1853023/1‑69 (MQ=255) acaacCGTGGCAGTTCGTGCTGCACATGAAGAGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTAt > 1:1686829/1‑71 (MQ=255) caacCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCa < 1:2649047/43‑1 (MQ=255) caacCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCa < 1:3546652/43‑1 (MQ=255) caacCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATc > 1:2136247/1‑71 (MQ=255) aacCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATAc > 1:2842790/1‑47 (MQ=255) aacCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATcc < 1:21783/71‑1 (MQ=255) acCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCa < 1:357538/71‑1 (MQ=255) tGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCtt > 1:3348018/1‑71 (MQ=255) ttCGTGCTGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTgg < 1:672558/71‑1 (MQ=255) ttCGTGCTGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTgg < 1:960297/71‑1 (MQ=255) ttCGTGCTGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTgg < 1:941397/71‑1 (MQ=255) tCGTGCAGCACATGAAGGGGTGATTGGGCAGATAc > 1:2835592/1‑35 (MQ=255) tCGTGCAGCACATGAAGGGGTGATTGGGCAGATAc > 1:3205355/1‑35 (MQ=255) tCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTg > 1:2325237/1‑69 (MQ=255) cGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAg > 1:2140936/1‑71 (MQ=255) cGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAg > 1:3210256/1‑71 (MQ=255) gTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGc < 1:3074971/71‑1 (MQ=255) | GCACGCCCGATTACTGGAACATGCCAGTTTACTCTGGCAGCCTGAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGC > W3110S.gb/1724392‑1724524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |