Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,726,822	G→A	15.9%	T175T (ACC→ACT)	ydhF ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,726,822	0	G	A	15.9%	100.7 / 10.8	44	T175T (ACC→ACT)	ydhF	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base A (3/4);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GTCGAGCGTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCA  >  W3110S.gb/1726756‑1726892                                                                   |                                                                       gTCGAGCGTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTaa                                                                    <  1:3826225/71‑1 (MQ=255)        gTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACgg                                                                >  1:3480685/1‑68 (MQ=255)            cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGt                                                         <  1:2516563/71‑1 (MQ=255)            cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCg                                                          >  1:969683/1‑70 (MQ=255)            cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCg                                                          >  1:706265/1‑70 (MQ=255)            cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGt                                                         >  1:1196775/1‑71 (MQ=255)            cATCCAGCAGTAACCGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGt                                                         <  1:2573090/71‑1 (MQ=255)             aTCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTg                                                        <  1:1751877/71‑1 (MQ=255)               ccAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAt                                                      >  1:3146483/1‑71 (MQ=255)                cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTg                                                        >  1:2823989/1‑68 (MQ=255)                cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                     >  1:3103761/1‑71 (MQ=255)                cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                     >  1:3820221/1‑71 (MQ=255)                cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                     >  1:1581690/1‑71 (MQ=255)                     gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCaa                                                 <  1:591315/70‑1 (MQ=255)                     gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCaa                                                 <  1:1686312/70‑1 (MQ=255)                     gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                <  1:2021153/71‑1 (MQ=255)                     gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                <  1:2351380/71‑1 (MQ=255)                     gTAACGGCTGATGCACCGGGGATATTTCCACCTAATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                <  1:3321284/71‑1 (MQ=255)                     gTAACGGCTGATGCACCGGGGATATTTCCACCTAATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                <  1:3316817/71‑1 (MQ=255)                       aaCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                              >  1:3634535/1‑71 (MQ=255)                        aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCa                                                              >  1:932697/1‑54 (MQ=255)                        aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                             >  1:1356121/1‑71 (MQ=255)                        aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                             >  1:3768754/1‑71 (MQ=255)                        aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                             >  1:1225091/1‑71 (MQ=255)                        aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                             >  1:1583160/1‑71 (MQ=255)                        aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                             >  1:1612744/1‑71 (MQ=255)                             tGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCaaa                                        >  1:3058394/1‑71 (MQ=255)                              gATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                <  1:3300530/62‑1 (MQ=255)                                tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                              >  1:3129755/1‑62 (MQ=255)                                tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                              >  1:2059796/1‑62 (MQ=255)                                tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCa                                          <  1:3549903/66‑1 (MQ=255)                                  cACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAAc                                                                  >  1:54744/1‑40 (MQ=255)                                   aCCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTgcg                                   >  1:619683/1‑70 (MQ=255)                                   aCCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTgcg                                   >  1:431074/1‑70 (MQ=255)                                       tggATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCa                                          <  1:188746/58‑1 (MQ=255)                                             tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGc                                                   <  1:208464/44‑1 (MQ=255)                                             tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAg                        <  1:2524352/71‑1 (MQ=255)                                                 cACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTc                     <  1:3101476/70‑1 (MQ=255)                                                      gATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACg               >  1:3752014/1‑71 (MQ=255)                                                            tGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTa                           <  1:3356140/53‑1 (MQ=255)                                                            tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGGAGGTGTAAAGTTTGATAca               <  1:1629351/65‑2 (MQ=255)                                                            tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATAca               <  1:1912753/65‑2 (MQ=255)                                                            tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATAca               <  1:67333/65‑2 (MQ=255)                                                              gCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt        <  1:1036599/70‑1 (MQ=255)                                                                   ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGc   >  1:3770242/1‑70 (MQ=255)                                                                   ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCa  <  1:1099468/71‑1 (MQ=255)                                                                   |                                                                       GTCGAGCGTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCA  >  W3110S.gb/1726756‑1726892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑