Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,726,828	C→T	12.7%	P173P (CCG→CCA)	ydhF ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,726,828	0	C	T	12.7%	118.4 / 7.6	47	P173P (CCG→CCA)	ydhF	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (3/3);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTT  >  W3110S.gb/1726763‑1726895                                                                  |                                                                    gTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACgg                                                                   >  1:3480685/1‑68 (MQ=255)     cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGt                                                            <  1:2516563/71‑1 (MQ=255)     cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCg                                                             >  1:969683/1‑70 (MQ=255)     cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCg                                                             >  1:706265/1‑70 (MQ=255)     cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGt                                                            >  1:1196775/1‑71 (MQ=255)     cATCCAGCAGTAACCGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGt                                                            <  1:2573090/71‑1 (MQ=255)      aTCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTg                                                           <  1:1751877/71‑1 (MQ=255)        ccAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAt                                                         >  1:3146483/1‑71 (MQ=255)         cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTg                                                           >  1:2823989/1‑68 (MQ=255)         cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                        >  1:3103761/1‑71 (MQ=255)         cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                        >  1:3820221/1‑71 (MQ=255)         cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                        >  1:1581690/1‑71 (MQ=255)              gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCaa                                                    <  1:591315/70‑1 (MQ=255)              gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCaa                                                    <  1:1686312/70‑1 (MQ=255)              gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                   <  1:2351380/71‑1 (MQ=255)              gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                   <  1:2021153/71‑1 (MQ=255)              gTAACGGCTGATGCACCGGGGATATTTCCACCTAATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                   <  1:3321284/71‑1 (MQ=255)              gTAACGGCTGATGCACCGGGGATATTTCCACCTAATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                   <  1:3316817/71‑1 (MQ=255)                aaCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                                 >  1:3634535/1‑71 (MQ=255)                 aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCa                                                                 >  1:932697/1‑54 (MQ=255)                 aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                >  1:1356121/1‑71 (MQ=255)                 aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                >  1:3768754/1‑71 (MQ=255)                 aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                >  1:1612744/1‑71 (MQ=255)                 aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                >  1:1225091/1‑71 (MQ=255)                 aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                >  1:1583160/1‑71 (MQ=255)                      tGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCaaa                                           >  1:3058394/1‑71 (MQ=255)                       gATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                   <  1:3300530/62‑1 (MQ=255)                         tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                                 >  1:3129755/1‑62 (MQ=255)                         tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                                 >  1:2059796/1‑62 (MQ=255)                         tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCa                                             <  1:3549903/66‑1 (MQ=255)                           cACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAAc                                                                     >  1:54744/1‑40 (MQ=255)                            aCCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTgcg                                      >  1:619683/1‑70 (MQ=255)                            aCCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTgcg                                      >  1:431074/1‑70 (MQ=255)                                tggATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCa                                             <  1:188746/58‑1 (MQ=255)                                      tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGc                                                      <  1:208464/44‑1 (MQ=255)                                      tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAg                           <  1:2524352/71‑1 (MQ=255)                                          cACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTc                        <  1:3101476/70‑1 (MQ=255)                                               gATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACg                  >  1:3752014/1‑71 (MQ=255)                                                     tGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTa                              <  1:3356140/53‑1 (MQ=255)                                                     tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGGAGGTGTAAAGTTTGATAca                  <  1:1629351/65‑2 (MQ=255)                                                     tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATAca                  <  1:67333/65‑2 (MQ=255)                                                     tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATAca                  <  1:1912753/65‑2 (MQ=255)                                                       gCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt           <  1:1036599/70‑1 (MQ=255)                                                            ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGc      >  1:3770242/1‑70 (MQ=255)                                                            ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCa     <  1:1099468/71‑1 (MQ=255)                                                             gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCa     <  1:488673/70‑1 (MQ=255)                                                                aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCATGCGTAAAGTTCGATACGCCAAAATGACGCACtt  <  1:2938379/70‑1 (MQ=255)                                                                aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACtt  <  1:3578733/70‑1 (MQ=255)                                                                  |                                                                    GTGCCATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTT  >  W3110S.gb/1726763‑1726895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑