Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,726,834	A→G	11.7%	R171R (CGT→CGC)	ydhF ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,726,834	0	A	G	11.7%	128.0 / 6.6	51	R171R (CGT→CGC)	ydhF	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (21/24);  new base G (3/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT  >  W3110S.gb/1726767‑1726904                                                                    |                                                                       cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGt                                                                     <  1:2516563/71‑1 (MQ=255) cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCg                                                                      >  1:969683/1‑70 (MQ=255) cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCg                                                                      >  1:706265/1‑70 (MQ=255) cATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGt                                                                     >  1:1196775/1‑71 (MQ=255) cATCCAGCAGTAACCGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGt                                                                     <  1:2573090/71‑1 (MQ=255)  aTCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTg                                                                    <  1:1751877/71‑1 (MQ=255)    ccAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAt                                                                  >  1:3146483/1‑71 (MQ=255)     cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTg                                                                    >  1:2823989/1‑68 (MQ=255)     cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                                 >  1:3103761/1‑71 (MQ=255)     cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                                 >  1:3820221/1‑71 (MQ=255)     cagcagTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGAtt                                                                 >  1:1581690/1‑71 (MQ=255)          gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCaa                                                             <  1:1686312/70‑1 (MQ=255)          gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCaa                                                             <  1:591315/70‑1 (MQ=255)          gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                            <  1:2021153/71‑1 (MQ=255)          gTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                            <  1:2351380/71‑1 (MQ=255)          gTAACGGCTGATGCACCGGGGATATTTCCACCTAATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                            <  1:3321284/71‑1 (MQ=255)          gTAACGGCTGATGCACCGGGGATATTTCCACCTAATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                            <  1:3316817/71‑1 (MQ=255)            aaCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                                          >  1:3634535/1‑71 (MQ=255)             aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                         >  1:1225091/1‑71 (MQ=255)             aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                         >  1:1612744/1‑71 (MQ=255)             aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                         >  1:1583160/1‑71 (MQ=255)             aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                         >  1:3768754/1‑71 (MQ=255)             aCGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAgg                                                         >  1:1356121/1‑71 (MQ=255)                  tGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCaaa                                                    >  1:3058394/1‑71 (MQ=255)                   gATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAAc                                                            <  1:3300530/62‑1 (MQ=255)                     tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                                          >  1:2059796/1‑62 (MQ=255)                     tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAg                                                          >  1:3129755/1‑62 (MQ=255)                     tGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCa                                                      <  1:3549903/66‑1 (MQ=255)                        aCCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTgcg                                               >  1:619683/1‑70 (MQ=255)                        aCCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTgcg                                               >  1:431074/1‑70 (MQ=255)                            tggATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCa                                                      <  1:188746/58‑1 (MQ=255)                                  tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGc                                                               <  1:208464/44‑1 (MQ=255)                                  tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAg                                    <  1:2524352/71‑1 (MQ=255)                                      cACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTc                                 <  1:3101476/70‑1 (MQ=255)                                           gATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACg                           >  1:3752014/1‑71 (MQ=255)                                                 tGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTa                                       <  1:3356140/53‑1 (MQ=255)                                                 tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGGAGGTGTAAAGTTTGATAca                           <  1:1629351/65‑2 (MQ=255)                                                 tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATAca                           <  1:67333/65‑2 (MQ=255)                                                 tGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATAca                           <  1:1912753/65‑2 (MQ=255)                                                   gCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                    <  1:1036599/70‑1 (MQ=255)                                                        ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGc               >  1:3770242/1‑70 (MQ=255)                                                        ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCa              <  1:1099468/71‑1 (MQ=255)                                                         gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCa              <  1:488673/70‑1 (MQ=255)                                                            aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCATGCGTAAAGTTCGATACGCCAAAATGACGCACtt           <  1:2938379/70‑1 (MQ=255)                                                            aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACtt           <  1:3578733/70‑1 (MQ=255)                                                                gCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATCCGCCaa                       <  1:4949/54‑1 (MQ=255)                                                                  aGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGct    >  1:3111610/1‑71 (MQ=255)                                                                  aGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGct    >  1:444642/1‑71 (MQ=255)                                                                  aGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGct    >  1:1993950/1‑71 (MQ=255)                                                                   gACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGctc   <  1:1376075/71‑1 (MQ=255)                                                                   gACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGctc   <  1:963362/71‑1 (MQ=255)                                                                    aCGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGctct  >  1:3706948/1‑71 (MQ=255)                                                                    |                                                                       CATCCAGCAGTAACGGCTGATGCACCGGGGATATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT  >  W3110S.gb/1726767‑1726904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑