| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,739,599 | C→A | 13.4% | S14S (TCC→TCA) | purR → | DNA‑binding transcriptional repressor, hypoxanthine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,739,599 | 0 | C | A | 13.4% | 105.2 / 3.4 | 37 | S14S (TCC→TCA) | purR | DNA‑binding transcriptional repressor, hypoxanthine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (18/14); new base A (3/2); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
GAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTG > W3110S.gb/1739532‑1739667 | gAAGTTAGGGTCTTGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCAc > 1:2510302/1‑70 (MQ=255)gAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCAc > 1:1068069/1‑70 (MQ=255)gAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCAc > 1:2228509/1‑70 (MQ=255)gAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCAc > 1:153581/1‑70 (MQ=255)gAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCAc > 1:423057/1‑70 (MQ=255) tGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTca > 1:2080933/1‑71 (MQ=255) gAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTcaca > 1:3326793/1‑71 (MQ=255) aGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCAACTACAACTGTGTCacac < 1:420422/71‑1 (MQ=255) aGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCAACTACAACTGTGTCacac < 1:206928/71‑1 (MQ=255) atggCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATcaacaa < 1:2154695/71‑1 (MQ=255) atggCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATcaacaa < 1:75638/71‑1 (MQ=255) gCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACaaaa > 1:2400994/1‑70 (MQ=255) gCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACaaaa > 1:2624992/1‑70 (MQ=255) aaTAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTc > 1:1844104/1‑48 (MQ=255) aaaaGATGTAGCGAAACGAGCAAACGTTTCCACTACAAc > 1:1247728/1‑39 (MQ=255) aGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTtcgtcg < 1:748520/71‑1 (MQ=255) gATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGc < 1:2792965/71‑1 (MQ=255) tGTAGCGAAACGAGCAAACGTTTCCACTACAACTATGTCACACGTGATCAACAAAACACGTTTCGTCGCTg < 1:3759795/71‑1 (MQ=255) tAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTga < 1:319092/70‑1 (MQ=255) gCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaaga < 1:1106458/71‑1 (MQ=255) gCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaaga < 1:3430651/71‑1 (MQ=255) cGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAaca > 1:225731/1‑48 (MQ=255) cGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAAcgcg < 1:205667/71‑1 (MQ=255) gAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACgcgc < 1:2236462/71‑1 (MQ=255) aCGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGt > 1:1234116/1‑44 (MQ=255) aCGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGt > 1:3580568/1‑44 (MQ=255) aCGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGcc < 1:3620363/71‑1 (MQ=255) cGTTTCAACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAATGCCg > 1:3706926/1‑71 (MQ=255) cGTTTCAACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAATGCCg > 1:2035711/1‑71 (MQ=255) cGTTTCAACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAATGCCg > 1:3182067/1‑71 (MQ=255) gTTTCCACTACAACTGTGTCACACGTGATCAACAAAaca > 1:3201786/1‑39 (MQ=255) gTTTCCACTACAACTGTGTCACACGTGATCAACAAAaca > 1:643253/1‑39 (MQ=255) gTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCgt > 1:427456/1‑71 (MQ=255) tCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaagaa < 1:3176352/54‑1 (MQ=255) tCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaagaa < 1:532597/54‑1 (MQ=255) tCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaagaa < 1:1835937/54‑1 (MQ=255) tCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGtgtg > 1:634196/1‑71 (MQ=255) cACTACAACTGTGTCACACGTGATCAACAAAACACGttt > 1:3239948/1‑39 (MQ=255) | GAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTG > W3110S.gb/1739532‑1739667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |