| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,927,471 | T→A | 9.3% | P106P (CCT→CCA) | yobB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,927,471 | 0 | T | A | 9.3% | 140.4 / 6.8 | 54 | P106P (CCT→CCA) | yobB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (28/21); new base A (3/2); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
TCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCGATGAA > W3110S.gb/1927405‑1927540 | tCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGa > 1:3734067/1‑71 (MQ=255) cccGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGa > 1:1610516/1‑70 (MQ=255) cccGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGAt > 1:1442778/1‑71 (MQ=255) ccGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATc > 1:3767696/1‑71 (MQ=255) ccGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATc > 1:2048796/1‑71 (MQ=255) cGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATc > 1:1633867/1‑70 (MQ=255) gTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTa > 1:2141317/1‑71 (MQ=255) gAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGAt > 1:1359812/1‑65 (MQ=255) gAATATAACGATCGCTTTATACGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCa > 1:2866111/1‑71 (MQ=255) aaTATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCAt < 1:3122141/71‑1 (MQ=255) aaTATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCAt < 1:2888408/71‑1 (MQ=255) ataACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCAt > 1:3825970/1‑68 (MQ=255) ataACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCAt > 1:3622702/1‑68 (MQ=255) aCGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCt > 1:2384669/1‑53 (MQ=255) gATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCa > 1:922817/1‑71 (MQ=255) aTCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATc < 1:258231/63‑1 (MQ=255) aTCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAt < 1:615304/71‑1 (MQ=255) aTCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAt < 1:528390/71‑1 (MQ=255) tCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTggg < 1:232377/52‑1 (MQ=255) tCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTggg < 1:1871475/52‑1 (MQ=255) gCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATg > 1:2232979/1‑69 (MQ=255) ttATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGc < 1:3365099/70‑1 (MQ=255) tATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGGTCTACCATCAGAGTCATGGTGc < 1:3674819/69‑1 (MQ=255) tATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGc < 1:2086083/69‑1 (MQ=255) tATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCAt > 1:1136390/1‑71 (MQ=255) tATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCAt > 1:847286/1‑71 (MQ=255) tATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCAt > 1:1751288/1‑71 (MQ=255) tGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCcgtc > 1:2837209/1‑71 (MQ=255) tGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCcgtc > 1:2841839/1‑71 (MQ=255) tgtTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGt > 1:1810873/1‑44 (MQ=255) cGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGaa > 1:2127498/1‑71 (MQ=255) cGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGaa > 1:1602468/1‑71 (MQ=255) cGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGaa > 1:330852/1‑71 (MQ=255) cGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGa < 1:3077828/70‑1 (MQ=255) ccGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTg < 1:1397984/51‑1 (MQ=255) gTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCa > 1:1302973/1‑68 (MQ=255) gTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCa < 1:501694/71‑1 (MQ=255) tGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCa < 1:2961525/70‑1 (MQ=255) tGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCAc < 1:1917853/71‑1 (MQ=255) gCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACgg < 1:2412206/71‑1 (MQ=255) gCGAAAAACGCCTGGGATCTACAATCAGAGTCATGGTGCATGTCTGGGACGTCGTTCCAGAACCATCACgg > 1:874922/1‑71 (MQ=255) gAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACggtg > 1:50938/1‑71 (MQ=255) gAAAAACGCCTGGGATCTACCATCAGAGTCATGGAGCATGTCTGGGCCGTCGTTCCAGAACCATCACggtg > 1:283765/1‑71 (MQ=255) gAAAAACGCCAGGGATCTACCATCAGAGTTATGGt < 1:851643/35‑1 (MQ=37) gAAAAACGCCAGGGATCTACCATCAGAGTTATGGt < 1:13170/35‑1 (MQ=37) aaaaCGCCAGGGATCTACCATCAGAGTTATGGTGCATGTCTGGGCCGTCGTTcc > 1:613548/1‑54 (MQ=255) aaaaCGCCAGGGATCTACCATCAGAGTTATGGTGCATGTCTGGGCCGTCGTTcc > 1:2270927/1‑54 (MQ=255) aaaaCGCCAGGGATCTACCATCAGAGTTATGGTGCATGTCTGGGCCGTCGTTcc > 1:836011/1‑54 (MQ=255) aaaCGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTg > 1:999990/1‑41 (MQ=255) aaaCGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTc < 1:1872771/71‑1 (MQ=255) aaCGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTc > 1:3579386/1‑70 (MQ=255) aaCGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCg < 1:444366/71‑1 (MQ=255) aaCGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCg < 1:1085933/71‑1 (MQ=255) cGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCGAt < 1:2363272/71‑1 (MQ=255) cTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCGATGaa < 1:3692656/71‑1 (MQ=255) tGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCGATGaa > 1:1990153/1‑70 (MQ=255) | TCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGTCTGGGCCGTCGTTCCAGAACCATCACGGTGGTCGATGAA > W3110S.gb/1927405‑1927540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |