Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,381,930 Δ1 bp 100% coding (333/759 nt) yfbB ← predicted peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,381,9300C.92.9% 43.4 / ‑1.3 14coding (333/759 nt)yfbBpredicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (2/11);  total (2/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCG  >  W3110S.gb/2381869‑2381961
                                                             |                               
cccATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                         <  1:596067/69‑1 (MQ=255)
     tGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCGGCCTTCGACa                     <  1:3224176/69‑1 (MQ=255)
      ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAAt                   <  1:1221501/69‑1 (MQ=255)
      ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAAt                   <  1:1357010/69‑1 (MQ=255)
      ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGACCCGGATG‑CCGCCTTCGACAAt                   <  1:1256234/69‑1 (MQ=255)
          aTCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAAcc               <  1:2128418/69‑1 (MQ=255)
            cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACaa                    <  1:1318449/62‑1 (MQ=255)
            cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACaa                    >  1:1633037/1‑62 (MQ=255)
            cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACaa                    >  1:643793/1‑62 (MQ=255)
            cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACaa                    <  1:702107/62‑1 (MQ=255)
            cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACaa                    <  1:736161/62‑1 (MQ=255)
              gAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACaa                    <  1:2328696/60‑1 (MQ=255)
                aCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCcaca          <  1:509653/68‑1 (MQ=255)
                        gTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCg  <  1:877455/68‑1 (MQ=255)
                                                             |                               
CCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCG  >  W3110S.gb/2381869‑2381961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑