| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,225,032 | Δ1 bp | 100% | coding (143/1434 nt) | ygjI → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,225,032 | 0 | T | . | 84.6% | 74.9 / 12.3 | 26 | coding (143/1434 nt) | ygjI | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base . (11/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAA > W3110S.gb/3224964‑3225096 | tAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAt > 1:610737/1‑69 (MQ=255) aacaacGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtt > 1:3365265/1‑69 (MQ=255) acGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAttttta > 1:1237654/1‑68 (MQ=255) acGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAttttta > 1:868446/1‑68 (MQ=255) aataataatATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTc > 1:1082943/1‑69 (MQ=255) aataataatATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTc > 1:1838409/1‑69 (MQ=255) aataataatATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTc > 1:1936963/1‑69 (MQ=255) taataataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTccc < 1:222010/69‑1 (MQ=255) aataataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCt < 1:2734186/69‑1 (MQ=255) ataataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtttttat > 1:1843898/1‑56 (MQ=255) gAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctgtctg < 1:1630300/69‑1 (MQ=255) gAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTACCTtc > 1:2816216/1‑63 (MQ=255) ttGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGatcat > 1:1400227/1‑69 (MQ=255) ttGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGatcat > 1:2112134/1‑69 (MQ=255) tGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGatcat > 1:197717/1‑68 (MQ=255) ggACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtttt < 1:1196286/41‑1 (MQ=255) ggACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGatcat > 1:842383/1‑68 (MQ=255) ggACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTc < 1:2565922/51‑1 (MQ=255) ggACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTc < 1:1731107/51‑1 (MQ=255) ggCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGtt > 1:1750520/1‑69 (MQ=255) aCCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTtct < 1:2187006/43‑1 (MQ=39) aCCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTtct < 1:2575373/43‑1 (MQ=39) aCCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTtct < 1:1496142/43‑1 (MQ=39) aCCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCg > 1:879532/1‑55 (MQ=255) gATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTta > 1:3624938/1‑69 (MQ=255) tGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaa > 1:1516651/1‑68 (MQ=255) tttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaataaa < 1:215280/69‑1 (MQ=255) tttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaataaa < 1:153167/69‑1 (MQ=255) cgcgACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCa < 1:2064521/69‑1 (MQ=255) cgcgACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCa < 1:1880498/69‑1 (MQ=255) gcgACGA‑TTTTTATTTTATTCACTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTc > 1:2485621/1‑67 (MQ=255) acgaTTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGaa > 1:3104383/1‑69 (MQ=255) | TAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAA > W3110S.gb/3224964‑3225096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |