| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,777,419:1 | +A | 100% | coding (3964/4581 nt) | ypjA ← | adhesin‑like autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,777,419 | 1 | . | A | 86.7% | 115.1 / 15.9 | 45 | coding (3964/4581 nt) | ypjA | adhesin‑like autotransporter |
| Reads supporting (aligned to +/- strand): ref base . (2/4); new base A (10/29); total (12/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATC > W3110S.gb/2777354‑2777485 | ttCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaa > 1:2811165/1‑69 (MQ=255) tCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaa > 1:2142565/1‑68 (MQ=255) cATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaaa > 1:2800152/1‑68 (MQ=255) aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaaaa > 1:3284173/1‑67 (MQ=255) aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaaaa > 1:2067013/1‑67 (MQ=255) aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaaa < 1:1563876/67‑1 (MQ=255) tGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaaaac < 1:1852333/68‑3 (MQ=255) tGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑aaaaac < 1:1930606/68‑3 (MQ=255) gTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGa < 1:1424397/69‑1 (MQ=255) ttCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑a > 1:2934341/1‑61 (MQ=255) aaCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATAtgtg < 1:173066/69‑1 (MQ=255) aCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:465049/69‑1 (MQ=255) aCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:1494336/69‑1 (MQ=255) aCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:1762877/69‑1 (MQ=255) aTAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATg < 1:2230108/67‑1 (MQ=255) aTAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATg < 1:1713309/67‑1 (MQ=255) aTAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATg < 1:118983/67‑1 (MQ=255) tAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATg < 1:3653466/66‑1 (MQ=255) tAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGa < 1:3174470/69‑1 (MQ=255) tAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGa < 1:2614926/69‑1 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:3542582/60‑1 (MQ=255) cAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATggg > 1:3238376/1‑60 (MQ=255) cAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATggg > 1:202616/1‑60 (MQ=255) gaATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:2455141/50‑1 (MQ=255) gaATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:3659112/50‑1 (MQ=255) gaATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:2708942/50‑1 (MQ=255) gaATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATaaa > 1:2451976/1‑69 (MQ=255) aaTAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAg > 1:15855/1‑69 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATggg < 1:2162677/51‑1 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATggg < 1:269259/51‑1 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATggg < 1:1207182/51‑1 (MQ=255) cTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATaaa > 1:3172732/1‑63 (MQ=255) cTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATaaa > 1:701123/1‑63 (MQ=255) cTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:1073237/35‑1 (MQ=37) cTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:3405133/35‑1 (MQ=37) cTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg > 1:3195683/1‑35 (MQ=37) cTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:3678702/69‑1 (MQ=255) tGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:663140/68‑1 (MQ=255) tGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt > 1:898781/1‑68 (MQ=255) ccATGTCCTCCGCGATCAAAAACCGATATGTGAATggg < 1:3128908/38‑1 (MQ=38) ccATGTCCTCCGCGATCAAAAACCGATATGTGAATggg < 1:2686176/38‑1 (MQ=38) ccATGTCCTCCGCGATCAAAAACCGATATGTGAATggg < 1:732309/38‑1 (MQ=38) ctGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAAt < 1:1964948/66‑1 (MQ=255) aTGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAAt < 1:1470763/67‑1 (MQ=255) aTGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAAt < 1:2145982/67‑1 (MQ=255) tcctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATa > 1:3188072/1‑44 (MQ=38) tcctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATa > 1:494145/1‑44 (MQ=38) ctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTc < 1:684843/68‑1 (MQ=255) cgcgATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAgg > 1:1400860/1‑68 (MQ=255) cgATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAgg < 1:2452238/66‑1 (MQ=255) cgATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAgg < 1:2334426/66‑1 (MQ=255) gATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat < 1:437714/69‑1 (MQ=255) gATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat < 1:2381317/69‑1 (MQ=255) tC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATc > 1:2211752/1‑68 (MQ=255) | TTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATC > W3110S.gb/2777354‑2777485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |