| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,428,262:1 | +G | 100% | coding (735/855 nt) | ulaE → | L‑xylulose 5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,428,262 | 1 | . | G | 81.8% | 57.7 / 6.6 | 22 | coding (735/855 nt) | ulaE | L‑xylulose 5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (10/8); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGC > W3110S.gb/4428196‑4428321 | gCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑gg > 1:600047/1‑69 (MQ=255) ttGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGt < 1:896895/69‑1 (MQ=255) gAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACTGAGTGGCTATTGCGGGGCCGTACCt < 1:3422130/69‑1 (MQ=255) gAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:1770919/69‑1 (MQ=255) gAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:2525841/69‑1 (MQ=255) gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt > 1:1891476/1‑69 (MQ=255) aTTTCGGACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGc > 1:472795/1‑44 (MQ=255) gAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGa < 1:1272070/68‑1 (MQ=255) aaCGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGaa > 1:656110/1‑69 (MQ=255) cGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt > 1:3092848/1‑48 (MQ=255) cGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt > 1:867537/1‑48 (MQ=255) cGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt > 1:3146197/1‑48 (MQ=255) cGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt > 1:1131119/1‑48 (MQ=255) cGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTgg < 1:405002/61‑1 (MQ=255) gttTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:1231977/44‑1 (MQ=38) tCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAg < 1:3639599/69‑1 (MQ=255) cGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGAccc < 1:2637992/68‑1 (MQ=255) cGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATCTGGAGCGAAACGGCGGAAGAccc < 1:1550100/68‑1 (MQ=255) gCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCcggc > 1:256109/1‑69 (MQ=255) cTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAAc > 1:2885949/1‑54 (MQ=38) aCAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAg < 1:663039/69‑1 (MQ=255) gTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTg > 1:3709576/1‑69 (MQ=255) gTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTg > 1:1595906/1‑69 (MQ=255) gCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGCAACGGCGGAAGACCCGGCGGCAGAAGTGGc > 1:1322617/1‑68 (MQ=255) | GCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGC > W3110S.gb/4428196‑4428321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |