| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,495,966:1 | +T | 73.2% | coding (820/924 nt) | ydcI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,495,966 | 1 | . | T | 73.2% | 78.1 / 37.3 | 41 | coding (820/924 nt) | ydcI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (8/3); new base T (20/10); total (28/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 | |||||||||||
AACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAG > W3110S.gb/1495899‑1496025 | aaCTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑t > 1:1025069/1‑69 (MQ=255)aaCTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑t > 1:48457/1‑69 (MQ=255) cTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑tt < 1:2286399/68‑2 (MQ=255) ggCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑Ttcc > 1:810936/1‑68 (MQ=255) ggCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑Ttcc > 1:2009459/1‑68 (MQ=255) ggCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑Ttcc > 1:1934173/1‑68 (MQ=255) cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAca < 1:1469807/69‑1 (MQ=255) cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAca > 1:1969900/1‑69 (MQ=255) aagaGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑Tcc < 1:2459518/58‑1 (MQ=255) gagaACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcaggcag > 1:2576584/1‑69 (MQ=255) gagaACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcaggcag > 1:2110125/1‑69 (MQ=255) gagaACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcaggcag > 1:278700/1‑69 (MQ=255) aCGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGcc < 1:549496/69‑1 (MQ=255) gCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑ttc > 1:539062/1‑45 (MQ=255) gCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑ttc > 1:211089/1‑45 (MQ=255) tACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGc > 1:3700025/1‑69 (MQ=255) gggTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTgg < 1:1464954/64‑1 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAgg > 1:3458923/1‑68 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt > 1:351065/1‑69 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt > 1:3030542/1‑69 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt > 1:3606897/1‑69 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt > 1:1648064/1‑69 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtc < 1:2439149/69‑1 (MQ=255) tATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtc < 1:1270157/69‑1 (MQ=255) ttCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGc > 1:1372728/1‑63 (MQ=255) ccAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtcgt < 1:3386295/68‑1 (MQ=255) ccAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtcgt < 1:1341854/68‑1 (MQ=255) ccAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCt > 1:40751/1‑69 (MQ=255) ccAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCt > 1:3277131/1‑69 (MQ=255) tCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtcg > 1:969030/1‑63 (MQ=255) cGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCttt > 1:1024555/1‑67 (MQ=255) ggTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTAc > 1:732572/1‑69 (MQ=255) ggTTCGCCGGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCAAGACGCAGgtcg > 1:2046036/1‑60 (MQ=255) tCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAgc > 1:2345050/1‑69 (MQ=255) tCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAgc > 1:2299136/1‑69 (MQ=255) tCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAgc > 1:2193757/1‑69 (MQ=255) tCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAgc > 1:1547428/1‑69 (MQ=255) tGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:1165793/69‑1 (MQ=255) tGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:957238/69‑1 (MQ=255) cACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtcgtc > 1:2510581/1‑53 (MQ=255) cACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtcgtc > 1:494087/1‑53 (MQ=255) cACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtttt < 1:2400207/57‑1 (MQ=255) cACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtttt < 1:2078216/57‑1 (MQ=255) cACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtttt < 1:611518/57‑1 (MQ=255) cACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCttt > 1:213015/1‑55 (MQ=255) ccATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtttt > 1:1556941/1‑55 (MQ=255) | AACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAG > W3110S.gb/1495899‑1496025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |