Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262331 3262332 2–1199 72 [0] [0] 68 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)51 (1.500) 24/92 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

ATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261063‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT  >  W3110S.gb/3262333‑3262399
                                                                                                                                          
ATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       <  1:2834809/69‑1
ATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       >  1:3363231/1‑69
 TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       >  1:2287682/1‑68
 TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       <  1:723123/68‑1
  CTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                     >  1:2945150/1‑69
   TACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    >  1:2245569/1‑69
       TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACT                                                                   <  1:1675789/66‑1
       TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACT                                                                   <  1:117603/66‑1
       TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    <  1:360566/65‑1
       TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                     >  1:2414357/1‑64
        TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTG                                                                >  1:1149440/1‑68
        TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTG                                                                >  1:1479150/1‑68
        TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                     >  1:737696/1‑63
        TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                     >  1:1518803/1‑63
         CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTA                                                                      >  1:928477/1‑61
         CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTA                                                                      >  1:14752/1‑61
          ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                              >  1:1297452/1‑68
            GAAAAATAATCTCTCTATAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                            <  1:2464507/68‑1
            GAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                            <  1:1686259/68‑1
             AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCT                                                             >  1:2598844/1‑66
                AATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:170979/68‑1
                   AATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                    <  1:2913846/69‑1
                   AATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAAT                                                     >  1:1089498/1‑68
                   AATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAAT                                                     >  1:1886784/1‑68
                           TACAATACTTCAACTAAATTATGCAAATTCTGGGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAA                                            >  1:2530366/1‑69
                           TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAA                                            >  1:3204448/1‑69
                           TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        >  1:2924901/1‑57
                           TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                         <  1:822299/56‑1
                           TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       <  1:2076870/42‑1
                           TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       <  1:2355654/42‑1
                            ACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAA                                            >  1:2950241/1‑68
                            ACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAA                                            >  1:300753/1‑68
                            ACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCA                                             >  1:2476021/1‑67
                             CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAA                                           >  1:1786011/1‑68
                             CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        >  1:271947/1‑55
                               ATACTTCAACTAAATTATGCAACTTCTGCGGGCGCGTTAACTTTGCCTA                                                            <  1:1287054/49‑1
                               ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                         <  1:779713/68‑1
                               ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                         <  1:312203/68‑1
                               ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                            <  1:120796/49‑1
                                TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCA                                        >  1:1999434/1‑68
                                TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                            <  1:595294/48‑1
                                TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    >  1:3132817/1‑40
                                TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    >  1:2010003/1‑40
                                 ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        >  1:1293094/1‑51
                                 ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        >  1:1124400/1‑51
                                 ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                 >  1:3453773/1‑42
                                       ACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                  <  1:3627841/67‑1
                                        CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                <  1:3023579/68‑1
                                         TAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTT                               <  1:3409278/68‑1
                                         TAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                <  1:721249/67‑1
                                          AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                  >  1:2878236/1‑64
                                          AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATC                                               <  1:1586295/51‑1
                                          AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        >  1:1272504/1‑42
                                          AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:3722222/42‑1
                                          AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        >  1:2678668/1‑42
                                          AAATTATGAAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                  >  1:1111680/1‑64
                                            ATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                <  1:2253261/64‑1
                                               ATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                        <  1:1191231/69‑1
                                               ATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                        <  1:1748970/69‑1
                                               ATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                        <  1:2424227/69‑1
                                               ATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                        <  1:178580/69‑1
                                               ATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                        <  1:352688/69‑1
                                                      TTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                      >  1:1253730/1‑64
                                                      TTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                      >  1:1670088/1‑64
                                                       TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                 >  1:2569097/1‑68
                                                        CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGA                >  1:2856966/1‑68
                                                        CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                 >  1:3445357/1‑67
                                                        CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAA                                           >  1:135442/1‑41
                                                        CTGCGGGCGCGTTAACTTTGCCAAAATGAATGGCATCCAAAGCAATCATTTTTT                              >  1:153488/1‑54
                                                          GCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT              <  1:842209/68‑1
                                                           CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT              <  1:206081/67‑1
                                                             GGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGGTAGTCGGAATAAC           >  1:490611/1‑68
                                                                    TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                <  1:3239868/40‑1
                                                                     AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT  >  1:368093/1‑69
                                                                     AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                  >  1:3613595/1‑37
                                                                     AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                  >  1:3446964/1‑37
                                                                      ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACA     <  1:2478798/65‑1
                                                                      ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACA     <  1:2193402/65‑1
                                                                      ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACA     <  1:2633717/65‑1
                                                                      ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACA     >  1:2685747/1‑65
                                                                                                                                          
ATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261063‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT  >  W3110S.gb/3262333‑3262399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.