| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,606,841 | C→T | 48.3% | A26V (GCC→GTC) | lsrB → | AI2 transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,606,841 | 0 | C | T | 48.3% | 1.7 / 36.6 | 29 | A26V (GCC→GTC) | lsrB | AI2 transporter |
| Reads supporting (aligned to +/- strand): ref base C (9/6); new base T (9/5); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCG > W3110S.gb/1606776‑1606897 | tCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCg < 1:17659/68‑1 (MQ=255) aGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCg < 1:950362/67‑1 (MQ=255) aGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCg < 1:3087226/67‑1 (MQ=255) aGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGAGCAGGCCGCAGAGCg < 1:1433210/67‑1 (MQ=255) aaaaTCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGTCGCAGAGCGTAt < 1:714852/68‑1 (MQ=255) cGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCAtt < 1:3677148/69‑1 (MQ=255) acttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTc > 1:2086228/1‑68 (MQ=255) taGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGcc > 1:2248804/1‑43 (MQ=255) aGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGTCGCAGAGCg > 1:1305833/1‑50 (MQ=255) cgcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCAAAc < 1:1197372/68‑1 (MQ=255) gcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTg > 1:1088400/1‑69 (MQ=255) gcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTg > 1:1872208/1‑69 (MQ=255) ggCATTGCCGCAATCTCTATGAATGTGCAGGTCGCAGAGCGTATTg > 1:82401/1‑46 (MQ=255) ggCATTGCCGCAATCTCTATGAATGTGCAGGTCGCAGAGCGTATTg > 1:1542424/1‑46 (MQ=255) ggCATTGCCGCAATCTCTATGAATGTGCAGGTCGCAGAGCGTATTg > 1:523641/1‑46 (MQ=255) gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGa > 1:1043575/1‑69 (MQ=255) gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGa > 1:570427/1‑69 (MQ=255) gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGa > 1:468544/1‑69 (MQ=255) gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGa > 1:3426677/1‑69 (MQ=255) gCCGCAATCTCTAAGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTggg > 1:1563691/1‑68 (MQ=255) aTCTCTATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAtttttt < 1:3163382/69‑1 (MQ=255) aTCTCTATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAtttttt < 1:160220/69‑1 (MQ=255) ctctATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCa > 1:3479923/1‑44 (MQ=255) ctctATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCa > 1:3481406/1‑44 (MQ=255) ctctATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCa > 1:1798813/1‑44 (MQ=255) ctctATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCa > 1:2199520/1‑44 (MQ=255) ctctATGAATGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCa > 1:18743/1‑44 (MQ=255) tGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCg > 1:3271506/1‑69 (MQ=255) aaTGTGCAGGTCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAttttt < 1:3237222/59‑1 (MQ=255) cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAttttt < 1:2893091/53‑1 (MQ=255) | TCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCG > W3110S.gb/1606776‑1606897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |