| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,620,710 | C→T | 83.8% | intergenic (‑88/‑124) | marC ← / → marR | predicted transporter/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,620,710 | 0 | C | T | 83.8% | 87.2 / 10.9 | 38 | intergenic (‑88/‑124) | marC/marR | predicted transporter/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base T (22/9); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGA > W3110S.gb/1620646‑1620774 | tGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCg > 1:637877/1‑69 (MQ=255) tCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGa > 1:994898/1‑68 (MQ=255) tCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGa > 1:2262809/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:355271/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:462126/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:865745/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:889856/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:1323939/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:1269656/1‑68 (MQ=255) aGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAc > 1:1262292/1‑68 (MQ=255) cGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAC‑aa < 1:1431290/68‑1 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGc > 1:759714/1‑68 (MQ=255) tAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTgg < 1:1547975/69‑1 (MQ=255) tAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑aa < 1:528204/42‑1 (MQ=255) tAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑aa < 1:2705380/42‑1 (MQ=255) tAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑aa < 1:2997694/42‑1 (MQ=255) aaGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCaaa > 1:1212867/1‑61 (MQ=255) aaGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCaa > 1:616312/1‑60 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCgg > 1:1420899/1‑68 (MQ=255) tCACTCTTTAGCTAGCCTTGGATCGCATTGAAC‑ATAACTTGAACCGATTTAGCAAAACGTGGCATCgg < 1:805558/68‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCgg > 1:3274959/1‑68 (MQ=255) tCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCgg < 1:938256/68‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc < 1:3225488/68‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc < 1:3512283/68‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc < 1:3074354/68‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc < 1:2908654/68‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc < 1:225708/68‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCa > 1:3713696/1‑69 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCa > 1:3078807/1‑69 (MQ=255) ctctTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTg > 1:2573636/1‑38 (MQ=255) ctctTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTg > 1:1840631/1‑38 (MQ=255) ctctTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTg > 1:414311/1‑38 (MQ=255) ctctTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTg > 1:3327173/1‑38 (MQ=255) tctTTAGCTAGCCTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCaa > 1:625905/1‑68 (MQ=255) cTTGTATCGCATTGAACAAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCAttt < 1:3521920/68‑1 (MQ=255) cTTGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCAttt < 1:1041047/67‑1 (MQ=255) ttGTATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGa > 1:2573182/1‑68 (MQ=255) gCATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGt > 1:913397/1‑41 (MQ=255) | TGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAAC‑AAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGA > W3110S.gb/1620646‑1620774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |