| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,692,839 | G→A | 77.4% | G425D (GGT→GAT) | ydgA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,692,839 | 0 | G | A | 77.4% | 120.1 / 35.0 | 62 | G425D (GGT→GAT) | ydgA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (5/9); new base A (34/14); total (39/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCA > W3110S.gb/1692783‑1692897 | gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAg < 1:2753230/68‑1 (MQ=255)gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAg < 1:3332067/68‑1 (MQ=255)gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGa < 1:1013584/69‑1 (MQ=255)gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGa < 1:1770270/69‑1 (MQ=255) cATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCa > 1:3087498/1‑69 (MQ=255) ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGc > 1:3172482/1‑68 (MQ=255) ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGc > 1:3386472/1‑68 (MQ=255) ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGc > 1:2960866/1‑68 (MQ=255) ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGc > 1:1512940/1‑68 (MQ=255) ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGc > 1:2276045/1‑68 (MQ=255) gATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaaaa > 1:2344041/1‑69 (MQ=255) gATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaaaa > 1:2513844/1‑69 (MQ=255) gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGc > 1:156983/1‑68 (MQ=255) gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGc > 1:1361830/1‑68 (MQ=255) aaCTGAGTTTATGACTCATGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa < 1:2363401/68‑1 (MQ=255) aCTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGaaa < 1:2094778/69‑1 (MQ=255) tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa > 1:806222/1‑65 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa > 1:765194/1‑64 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa < 1:3553997/64‑1 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa < 1:2904068/64‑1 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa > 1:1396573/1‑64 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:3597379/1‑68 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:342129/1‑68 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:1070045/1‑68 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:3612266/1‑68 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:3719894/1‑68 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:458913/1‑68 (MQ=255) gAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACa > 1:698035/1‑68 (MQ=255) tttATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa < 1:3428304/68‑1 (MQ=255) gACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTg < 1:2364011/68‑1 (MQ=255) gACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTg < 1:142905/68‑1 (MQ=255) aCTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa < 1:2026599/68‑1 (MQ=255) aCTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa < 1:2022433/68‑1 (MQ=255) cTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa < 1:2168847/67‑1 (MQ=255) tCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAgg < 1:2342535/69‑1 (MQ=255) aGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaa < 1:376137/40‑1 (MQ=255) aGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg > 1:1890226/1‑69 (MQ=255) aGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg > 1:1433996/1‑69 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:933790/1‑61 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:1316904/1‑61 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:1836623/1‑61 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:2947822/1‑61 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:2007498/1‑61 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:2622066/1‑61 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACCGGCGAAACAGCAAGTTGa > 1:1950117/1‑61 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa < 1:2695431/54‑1 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa < 1:2387921/54‑1 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATc > 1:3197029/1‑69 (MQ=255) aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATc > 1:2996733/1‑69 (MQ=255) aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATc > 1:3545780/1‑69 (MQ=255) gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa < 1:3073988/53‑1 (MQ=255) gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATc > 1:2566342/1‑68 (MQ=255) gAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGc < 1:2780618/69‑1 (MQ=255) aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa < 1:2420387/69‑1 (MQ=255) aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa < 1:2676766/69‑1 (MQ=255) aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa < 1:899352/69‑1 (MQ=255) cTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:2078183/1‑35 (MQ=255) cTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:2073953/1‑35 (MQ=255) cTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:620243/1‑35 (MQ=255) cTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:2968662/1‑35 (MQ=255) cTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:807180/1‑35 (MQ=255) cTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGa > 1:3679850/1‑53 (MQ=255) | GACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCA > W3110S.gb/1692783‑1692897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |