Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,775,080 | T→C | 73.3% | F285F (TTT→TTC) | ydiN → | predicted transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,775,080 | 0 | T | C | 73.3% | 48.5 / 17.3 | 31 | F285F (TTT→TTC) | ydiN | predicted transporter |
Reads supporting (aligned to +/- strand): ref base T (6/2); new base C (11/11); total (17/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
TCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCA > W3110S.gb/1775022‑1775144 | tCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTg < 1:2532709/66‑1 (MQ=255) tCTTTATTTTTGCCGCACAACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg < 1:3702627/66‑1 (MQ=255) tATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg > 1:3081962/1‑62 (MQ=255) tGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAc < 1:1739232/52‑1 (MQ=255) cGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCCAATGTATTTAACTCTGCACTGGCAACAATaa < 1:1028130/68‑1 (MQ=255) cACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAAc > 1:1138569/1‑67 (MQ=255) gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc < 1:717027/67‑1 (MQ=255) gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt < 1:154642/69‑1 (MQ=255) aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:1086710/67‑1 (MQ=255) aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:2933765/67‑1 (MQ=255) aTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGc > 1:2595255/1‑44 (MQ=255) gTCCGGCCCATCTTGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg < 1:1246272/69‑1 (MQ=255) tCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg < 1:3022519/68‑1 (MQ=255) cATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTtc < 1:407248/69‑1 (MQ=255) cTGGGCTAATGTTTTGAACTCTGCACTGGCAACAATAACAGCAGCCATTa < 1:919315/50‑1 (MQ=39) cTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTtctc > 1:673355/1‑68 (MQ=255) tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt > 1:3063535/1‑35 (MQ=255) tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt > 1:2322906/1‑35 (MQ=255) tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt > 1:750756/1‑35 (MQ=255) tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt > 1:886770/1‑35 (MQ=255) tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt > 1:1517001/1‑35 (MQ=255) tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt > 1:1081518/1‑35 (MQ=255) tGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCa > 1:199133/1‑69 (MQ=255) aTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGgtg < 1:2681838/68‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTa > 1:909455/1‑45 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTgg > 1:1708129/1‑65 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTg > 1:2116493/1‑64 (MQ=255) gTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGtgtg < 1:2004527/68‑1 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCa > 1:2371541/1‑69 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCa > 1:1914953/1‑69 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCa > 1:147962/1‑69 (MQ=255) tAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCt < 1:1610012/49‑1 (MQ=255) tAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCt < 1:1428142/49‑1 (MQ=255) | TCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCA > W3110S.gb/1775022‑1775144 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |