Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,775,080 T→C 73.3% F285F (TTT→TTC ydiN → predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,775,0800TC73.3% 48.5 / 17.3 31F285F (TTT→TTCydiNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base C (11/11);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCA  >  W3110S.gb/1775022‑1775144
                                                          |                                                                
tCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTg                                                           <  1:2532709/66‑1 (MQ=255)
tCTTTATTTTTGCCGCACAACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg                                                           <  1:3702627/66‑1 (MQ=255)
    tATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg                                                           >  1:3081962/1‑62 (MQ=255)
          tGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAc                                                               <  1:1739232/52‑1 (MQ=255)
             cGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCCAATGTATTTAACTCTGCACTGGCAACAATaa                                            <  1:1028130/68‑1 (MQ=255)
               cACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAAc                                           >  1:1138569/1‑67 (MQ=255)
                      gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                    <  1:717027/67‑1 (MQ=255)
                      gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt                                  <  1:154642/69‑1 (MQ=255)
                         aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt                                 <  1:1086710/67‑1 (MQ=255)
                         aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt                                 <  1:2933765/67‑1 (MQ=255)
                             aTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGc                                                    >  1:2595255/1‑44 (MQ=255)
                                gTCCGGCCCATCTTGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg                        <  1:1246272/69‑1 (MQ=255)
                                 tCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg                        <  1:3022519/68‑1 (MQ=255)
                                        cATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTtc                <  1:407248/69‑1 (MQ=255)
                                           cTGGGCTAATGTTTTGAACTCTGCACTGGCAACAATAACAGCAGCCATTa                                <  1:919315/50‑1 (MQ=39)
                                           cTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTtctc              >  1:673355/1‑68 (MQ=255)
                                            tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                              >  1:3063535/1‑35 (MQ=255)
                                            tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                              >  1:2322906/1‑35 (MQ=255)
                                            tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                              >  1:750756/1‑35 (MQ=255)
                                            tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                              >  1:886770/1‑35 (MQ=255)
                                            tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                              >  1:1517001/1‑35 (MQ=255)
                                            tGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                              >  1:1081518/1‑35 (MQ=255)
                                            tGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCa            >  1:199133/1‑69 (MQ=255)
                                                   aTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGgtg      <  1:2681838/68‑1 (MQ=255)
                                                    tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTa                            >  1:909455/1‑45 (MQ=255)
                                                    tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTgg        >  1:1708129/1‑65 (MQ=255)
                                                    tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTg         >  1:2116493/1‑64 (MQ=255)
                                                     gTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGtgtg    <  1:2004527/68‑1 (MQ=255)
                                                      tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCa  >  1:2371541/1‑69 (MQ=255)
                                                      tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCa  >  1:1914953/1‑69 (MQ=255)
                                                      tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCa  >  1:147962/1‑69 (MQ=255)
                                                          tAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCt                  <  1:1610012/49‑1 (MQ=255)
                                                          tAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCt                  <  1:1428142/49‑1 (MQ=255)
                                                          |                                                                
TCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCA  >  W3110S.gb/1775022‑1775144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: