| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,832,108 | C→T | 22.8% | T124T (ACG→ACA) | astA ← | arginine succinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,832,108 | 0 | C | T | 22.8% | 116.5 / 30.6 | 57 | T124T (ACG→ACA) | astA | arginine succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base T (6/7); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATA > W3110S.gb/1832040‑1832170 | aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGc > 1:1268490/1‑69 (MQ=255)aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGc > 1:2020592/1‑69 (MQ=255)aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGc > 1:3607805/1‑69 (MQ=255)aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGc > 1:3276880/1‑69 (MQ=255) cataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCTGGTCGAGAAACAGCg < 1:1552669/68‑1 (MQ=255) ataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTg < 1:1493127/69‑1 (MQ=255) taaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTg > 1:1253968/1‑68 (MQ=255) aaGCGCGATTTCGACAGCAAATAGCCGTTGCCGTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGcac > 1:257386/1‑69 (MQ=255) aaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGcac > 1:203256/1‑69 (MQ=255) aaGCGCGATTTCGACAGAAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGcac > 1:300551/1‑69 (MQ=255) cgcgATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGc > 1:2606336/1‑69 (MQ=255) cgATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTc < 1:2771880/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:1709177/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:2680550/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:695342/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:23750/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:2123913/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:437877/69‑1 (MQ=255) tttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgct < 1:604471/69‑1 (MQ=255) gCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCgg < 1:69978/69‑1 (MQ=255) aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgt > 1:1027948/1‑68 (MQ=255) aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgt > 1:2267079/1‑68 (MQ=255) aTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGtgtg < 1:1642711/69‑1 (MQ=255) tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGa > 1:267551/1‑69 (MQ=255) aGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTG‑‑CAGCTCGCTGCTGCCGGTGTGATc > 1:1348077/1‑68 (MQ=255) gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATc < 1:1838167/69‑1 (MQ=255) cGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGt > 1:3337943/1‑69 (MQ=255) cGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGt > 1:2129443/1‑69 (MQ=255) cGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGt > 1:1734891/1‑69 (MQ=255) cGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:1803761/69‑1 (MQ=255) cGTTGCCCTCTTTGCGCCAGGCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:2949082/69‑1 (MQ=255) ttGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt < 1:2076934/68‑1 (MQ=255) ttGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTa < 1:1813388/69‑1 (MQ=255) ttGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTa < 1:502509/69‑1 (MQ=255) gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTa > 1:2324912/1‑67 (MQ=255) gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTa > 1:1915411/1‑67 (MQ=255) tctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt > 1:3722912/1‑61 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga > 1:22571/1‑67 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga > 1:2227460/1‑67 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga > 1:2174575/1‑67 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga > 1:1548267/1‑67 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga > 1:383783/1‑67 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCg > 1:2122994/1‑40 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCg > 1:1561664/1‑40 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCg > 1:1762965/1‑40 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGtgtg > 1:1355018/1‑54 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGtgtg > 1:3686235/1‑54 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGtgtg > 1:1932867/1‑54 (MQ=255) tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGtgtg > 1:661467/1‑54 (MQ=255) gTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCgg < 1:3317668/69‑1 (MQ=255) tCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:418896/49‑1 (MQ=255) tCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:3216094/49‑1 (MQ=255) tCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:3543445/49‑1 (MQ=255) tCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:347009/49‑1 (MQ=255) tCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:1405942/49‑1 (MQ=255) tCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc < 1:442895/69‑1 (MQ=255) tCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGGTACTGAGAAACAGCGTCGGc < 1:1041973/69‑1 (MQ=255) cGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgtgt > 1:3231212/1‑41 (MQ=255) gaAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTta < 1:385687/68‑1 (MQ=255) gaAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTta < 1:831856/68‑1 (MQ=255) aaaCAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTtata < 1:3317996/69‑1 (MQ=255) | AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATA > W3110S.gb/1832040‑1832170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |