| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,838,873 | C→T | 22.2% | P363S (CCG→TCG) | ynjB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,838,873 | 0 | C | T | 22.2% | 71.6 / 16.9 | 36 | P363S (CCG→TCG) | ynjB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (14/14); new base T (5/3); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
TTCTCGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACC > W3110S.gb/1838805‑1838936 | ttCTCGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTgc > 1:287139/1‑69 (MQ=255)ttCTCGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTgc > 1:1853861/1‑69 (MQ=255) ctcGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTgccg > 1:3277045/1‑69 (MQ=255) ctcGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTgccg > 1:1355296/1‑69 (MQ=255) cGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTgccgcc > 1:2075275/1‑69 (MQ=255) tCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCgg < 1:1054492/68‑1 (MQ=255) cAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTg > 1:3147566/1‑69 (MQ=255) cAAAAACTGCATGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTg > 1:3218945/1‑69 (MQ=255) gACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACcgca > 1:430322/1‑68 (MQ=255) gggCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACcgcacg < 1:2738580/67‑1 (MQ=255) gcgAATCATTGCAATCAAGAATGCGGCAGGATCTGTCGCCGGt > 1:555962/1‑43 (MQ=38) gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt > 1:2568751/1‑43 (MQ=255) gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt > 1:1857348/1‑43 (MQ=255) gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTgg > 1:862723/1‑68 (MQ=255) gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTgg > 1:3599159/1‑68 (MQ=255) aaTCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTaa > 1:1549071/1‑69 (MQ=255) tCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACcgcacgca > 1:34318/1‑57 (MQ=255) tCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACcgcacgca > 1:1330638/1‑57 (MQ=255) aTTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGt < 1:3425561/36‑1 (MQ=255) aTTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGt < 1:662369/36‑1 (MQ=255) aTTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGt < 1:2549505/36‑1 (MQ=255) aTTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGt < 1:850892/36‑1 (MQ=255) aTTGCAATCAAGAATGCCGCAGGATCTCCCGCCGGt < 1:3101334/36‑1 (MQ=255) aaGAATGCCGCGGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGa > 1:1208861/1‑66 (MQ=255) aaGAATGCCGCGGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGa > 1:1204569/1‑66 (MQ=255) aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTg < 1:1853985/35‑1 (MQ=255) aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTg < 1:108777/35‑1 (MQ=255) aaGAATGCCGCAGGATCTGTCGCCGGTAATGGCTg < 1:3972/35‑1 (MQ=37) aaGAATGCCGCAGGATCTGCCGCCGGTACTGGCTg < 1:2255828/35‑1 (MQ=255) aaGAATGCCGCAGGATCTGCCGCCGGTACTGGCTg < 1:1663278/35‑1 (MQ=255) aaGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGa > 1:1468930/1‑66 (MQ=255) aaGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGa > 1:1079465/1‑66 (MQ=255) gAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:2523117/69‑1 (MQ=255) gAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:1333390/69‑1 (MQ=255) tGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:174952/66‑1 (MQ=255) gCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa < 1:3255259/49‑1 (MQ=255) ccGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATg > 1:3334109/1‑68 (MQ=255) ccGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATg > 1:1428455/1‑68 (MQ=255) gCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGc < 1:1354725/68‑1 (MQ=255) tCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACAcc > 1:1373516/1‑68 (MQ=255) tCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACAcc > 1:1105394/1‑68 (MQ=255) | TTCTCGATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACC > W3110S.gb/1838805‑1838936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |