Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,887,874	T→C	75.0%	F106L (TTT→CTT)	yeaX →	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,887,874	0	T	C	75.0%	118.4 / 39.9	64	F106L (TTT→CTT)	yeaX	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (8/8);  new base C (28/20);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

TCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCTTTC  >  W3110S.gb/1887808‑1887939                                                                   |                                                                  tCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGctt                                                                 >  1:2691919/1‑69 (MQ=255)  cccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGctt                                                                 >  1:3479270/1‑68 (MQ=255)  cccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTg                                                                >  1:1348502/1‑69 (MQ=255)     gCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTgcg                                                              >  1:1161194/1‑68 (MQ=255)       tttttGCATCAGCAGGTAAAAGTTGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTa                                                           <  1:3409863/69‑1 (MQ=255)       tttttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTa                                                           <  1:1508517/69‑1 (MQ=255)       tttttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTa                                                           <  1:3464106/69‑1 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCt                                                            <  1:152168/58‑1 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCt                                                            >  1:435604/1‑58 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa                                                  >  1:2422862/1‑68 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa                                                  >  1:2405153/1‑68 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa                                                  >  1:2796566/1‑68 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa                                                  >  1:3052923/1‑68 (MQ=255)                 agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa                                                  >  1:3283166/1‑68 (MQ=255)                        aaaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGaa                                           >  1:1059058/1‑68 (MQ=255)                        aaaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGaa                                           >  1:2193560/1‑68 (MQ=255)                        aaaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGaa                                           >  1:3692396/1‑68 (MQ=255)                         aaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCt                                                            <  1:959227/50‑1 (MQ=255)                         aaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTa                                                           <  1:615870/51‑1 (MQ=255)                           aGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCa                                       >  1:3611845/1‑69 (MQ=255)                               ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCt                                                            >  1:2284228/1‑44 (MQ=255)                               ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAtt                                                        >  1:709097/1‑48 (MQ=255)                               ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAtt                                                        >  1:1070562/1‑48 (MQ=255)                               ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAtt                                                        >  1:1482123/1‑48 (MQ=255)                               ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAtt                                                        >  1:225004/1‑48 (MQ=255)                                 cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGtt                                 <  1:696540/69‑1 (MQ=255)                                   aTCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTa                                                           <  1:2619407/41‑1 (MQ=255)                                    tCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTAt                              >  1:626525/1‑69 (MQ=255)                                    tCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTAt                              >  1:221543/1‑69 (MQ=255)                                     cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                            >  1:613865/1‑38 (MQ=255)                                      ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAg                                                 >  1:3628953/1‑48 (MQ=255)                                      ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                                >  1:915836/1‑49 (MQ=255)                                      ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                                >  1:2139035/1‑49 (MQ=255)                                      ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                                >  1:3285393/1‑49 (MQ=255)                                      ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcg                            >  1:3115333/1‑69 (MQ=255)                                         tAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgg                         >  1:847394/1‑69 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcggg                        >  1:1347773/1‑69 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcggg                        >  1:477368/1‑69 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGGATCTGttt                                <  1:555557/61‑1 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCAt                                      <  1:2600919/55‑1 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCAt                                      <  1:3464128/55‑1 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCAt                                      <  1:1707920/55‑1 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCAt                                      <  1:3497113/55‑1 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGttt                                >  1:3194932/1‑61 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGttt                                >  1:2250003/1‑61 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGttt                                >  1:262683/1‑61 (MQ=255)                                          aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcggg                        <  1:437951/69‑1 (MQ=255)                                           aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCAt                                      <  1:2685161/54‑1 (MQ=255)                                           aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCAt                                      <  1:1913556/54‑1 (MQ=255)                                            cGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggc                       <  1:3198911/68‑1 (MQ=255)                                            cGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggc                       <  1:3171777/68‑1 (MQ=255)                                             gATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg                     <  1:1666253/69‑1 (MQ=255)                                              aTTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGt                    >  1:2752804/1‑69 (MQ=255)                                                 tCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTAtt                 >  1:2421725/1‑69 (MQ=255)                                                     cGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGt              <  1:3390627/68‑1 (MQ=255)                                                       ccTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt            <  1:3546007/68‑1 (MQ=255)                                                       ccTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt            <  1:2827573/68‑1 (MQ=255)                                                       ccTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt            <  1:282682/68‑1 (MQ=255)                                                          aataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAcc        <  1:2036287/69‑1 (MQ=255)                                                           ataaCCTGTTTGCGCTAATTCCCTCAGCCATAAAGCa                                       <  1:422587/37‑1 (MQ=255)                                                           ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCa                                       <  1:1963402/37‑1 (MQ=255)                                                           ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCa                                       <  1:1651974/37‑1 (MQ=255)                                                              aCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCtt    >  1:2961954/1‑69 (MQ=255)                                                                cTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCttt   <  1:3316688/68‑1 (MQ=255)                                                                cTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCTTTc  >  1:2831672/1‑69 (MQ=255)                                                                cTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCTTTc  >  1:2310486/1‑69 (MQ=255)                                                                   |                                                                  TCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCTTTC  >  W3110S.gb/1887808‑1887939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑