| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,927,287 | T→C | 78.0% | L45P (CTT→CCT) | yobB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,927,287 | 0 | T | C | 78.0% | 60.2 / 13.1 | 32 | L45P (CTT→CCT) | yobB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base C (7/17); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACT > W3110S.gb/1927223‑1927347 | catcatCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttct < 1:2645623/69‑1 (MQ=255)catcatCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttc > 1:2554778/1‑68 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt > 1:2409684/1‑68 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:3234519/67‑1 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:2637122/67‑1 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:2568308/67‑1 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:949160/67‑1 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:2064218/67‑1 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGacttctt < 1:656741/67‑1 (MQ=255) catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTa < 1:1491234/69‑1 (MQ=255) atcTGGAGTTTGTGATGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt < 1:1289772/67‑1 (MQ=255) atcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt < 1:1150027/67‑1 (MQ=255) tcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:586564/65‑1 (MQ=255) ttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGtgtg < 1:2282139/69‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt < 1:441239/57‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt < 1:2160040/57‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt < 1:286728/57‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt < 1:2401232/57‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt < 1:251772/57‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttcttt < 1:1285206/57‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGtgtg > 1:2943944/1‑68 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGtgtg > 1:2998308/1‑68 (MQ=255) tgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGat < 1:3401351/68‑1 (MQ=255) tgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGat < 1:2570479/68‑1 (MQ=255) ggCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGattatt < 1:1890633/68‑1 (MQ=255) ggCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGattatt < 1:3518084/68‑1 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt > 1:1386492/1‑50 (MQ=255) ccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTg < 1:403374/69‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGat < 1:3588226/52‑1 (MQ=255) gCAACAATGTAAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:76913/69‑1 (MQ=255) aacaaTGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:1292211/36‑1 (MQ=255) aacaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt < 1:1308364/47‑1 (MQ=255) aaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGattat < 1:315636/50‑1 (MQ=255) aaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGc > 1:984410/1‑61 (MQ=255) aaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGc > 1:1785483/1‑61 (MQ=255) aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgcg > 1:396326/1‑54 (MQ=255) aTGTCAGCTGTTAGTTTTTCCATCGCATTCTTTACTGGGGTGTGATTATTcgcg > 1:1514420/1‑54 (MQ=255) cAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACg > 1:2172402/1‑53 (MQ=255) cAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg > 1:2173075/1‑55 (MQ=255) cAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg > 1:528297/1‑55 (MQ=255) gTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGa > 1:3266178/1‑69 (MQ=255) tAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtc > 1:2246874/1‑69 (MQ=255) gTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:3162588/50‑1 (MQ=255) gTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:556125/50‑1 (MQ=255) tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCa > 1:1391705/1‑69 (MQ=255) tCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc < 1:1217016/68‑1 (MQ=255) tCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:1868428/45‑1 (MQ=255) tCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:1735172/45‑1 (MQ=255) tCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:1510943/45‑1 (MQ=255) tCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACCTGCCCt < 1:473980/45‑1 (MQ=255) ccATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt < 1:2565896/68‑1 (MQ=255) ccATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt < 1:3101030/68‑1 (MQ=255) | CATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACT > W3110S.gb/1927223‑1927347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |