| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,934,313 | G→A | 19.0% | A53V (GCA→GTA) | eda ← | multifunctional 2‑keto‑3‑deoxygluconate 6‑phosphate aldolase, 2‑keto‑4‑hydroxyglutarate aldolase, and oxaloacetate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,934,313 | 0 | G | A | 19.0% | 44.2 / 5.2 | 21 | A53V (GCA→GTA) | eda | multifunctional 2‑keto‑3‑deoxygluconate 6‑phosphate aldolase, 2‑keto‑4‑hydroxyglutarate aldolase, and oxaloacetate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (12/5); new base A (2/2); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
TCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGG > W3110S.gb/1934245‑1934380 | tCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTg > 1:2965862/1‑69 (MQ=255)tCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTg > 1:2585457/1‑69 (MQ=255) ccatACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACt > 1:544488/4‑69 (MQ=255) ccatACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACt > 1:548736/4‑69 (MQ=255) acaATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTACACACTCGGTACGCAGAGTc > 1:2023977/1‑69 (MQ=255) acaATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTACACACTCGGTACGCAGAGTc > 1:2945220/1‑69 (MQ=255) aTCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACt < 1:3143028/69‑1 (MQ=255) aTCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACt < 1:2187108/69‑1 (MQ=255) cAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGaa > 1:2412813/1‑69 (MQ=255) aCTTCTTTGGCGATAGCACGGATAGCGTCAACTACACACTCGGTACGCAGAGTCACTTCCAGAACgcgc < 1:1378088/69‑1 (MQ=255) tcttTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAAcgcg < 1:1034330/65‑1 (MQ=255) cttTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAg > 1:471357/1‑58 (MQ=255) cttTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAg > 1:832065/1‑58 (MQ=255) cttTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAg < 1:2327105/58‑1 (MQ=255) cttTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAg < 1:1330771/58‑1 (MQ=255) cttTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCAccc > 1:2818514/1‑69 (MQ=255) ggCGATAGCACGGATAGCGTCAACTACACACTCGGTACGCAGAGTCACTTCCAGAACgcgc < 1:2729379/61‑1 (MQ=255) aGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGc > 1:2572504/1‑66 (MQ=255) cGGATAGCGTCAACTGCACACTCGGTACGCAGAGt > 1:1222546/1‑35 (MQ=255) cGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGcaaccaac > 1:2589541/1‑69 (MQ=255) cGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGcaaccaac > 1:2554941/1‑69 (MQ=255) gCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCtttt > 1:2653720/1‑69 (MQ=255) tGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCgg > 1:3721017/1‑69 (MQ=255) | TCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGG > W3110S.gb/1934245‑1934380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |