| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,995,272 | T→C | 69.2% | S524S (TCA→TCG) | uvrC ← | excinuclease UvrABC, endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,995,272 | 0 | T | C | 69.2% | 13.4 / 6.2 | 13 | S524S (TCA→TCG) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (4/5); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCC > W3110S.gb/1995207‑1995326 | cGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGa < 1:3597531/68‑1 (MQ=255) aTCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCaa > 1:1614308/1‑68 (MQ=255) cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTggcggc < 1:3253616/64‑1 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa < 1:1815702/69‑1 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa < 1:2369618/69‑1 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATcc < 1:790216/69‑1 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTc > 1:2342769/1‑69 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTc > 1:2767845/1‑69 (MQ=255) gtgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATcc > 1:2011236/1‑64 (MQ=255) gcgAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTaa > 1:2147449/1‑50 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCa < 1:3183080/68‑1 (MQ=255) aCATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcc > 1:3055892/1‑69 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCa < 1:573541/37‑1 (MQ=255) | CGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCC > W3110S.gb/1995207‑1995326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |