| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,999,934 | A→G | 48.7% | V6V (GTT→GTC) | yecC ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,999,934 | 0 | A | G | 48.7% | ‑6.6 / 54.5 | 37 | V6V (GTT→GTC) | yecC | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (12/7); new base G (6/12); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
GCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCT > W3110S.gb/1999870‑1999997 | gCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACtt < 1:1005508/69‑1 (MQ=255)gCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACtt < 1:2815668/69‑1 (MQ=255) tttACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCa > 1:1156995/1‑69 (MQ=255) tttACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCa > 1:388187/1‑69 (MQ=255) tttACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCa > 1:2820032/1‑69 (MQ=255) ccTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCa > 1:495178/1‑65 (MQ=255) ccTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCa < 1:1420786/65‑1 (MQ=255) cAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCa > 1:1545168/1‑68 (MQ=255) tACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTgg < 1:2620597/69‑1 (MQ=255) tACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTgg < 1:2616150/69‑1 (MQ=255) tACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTgg < 1:2559196/69‑1 (MQ=255) tACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTgg < 1:92550/69‑1 (MQ=255) tACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTgg < 1:1223620/69‑1 (MQ=255) aCCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTgg > 1:3312910/1‑68 (MQ=255) aCCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGt < 1:1008516/69‑1 (MQ=255) ccGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGt < 1:2848911/68‑1 (MQ=255) tCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGc < 1:3078938/68‑1 (MQ=255) ggaaaTTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTa < 1:2537854/62‑1 (MQ=255) gAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGa < 1:1356897/69‑1 (MQ=255) aaTTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGa < 1:700819/68‑1 (MQ=255) aCCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCaaaa < 1:1090787/68‑1 (MQ=255) ccAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAAt < 1:1708738/68‑1 (MQ=255) cAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATgg < 1:1319288/69‑1 (MQ=255) tCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGa < 1:3281444/52‑1 (MQ=255) tCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt > 1:88935/1‑68 (MQ=255) tCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt > 1:2028927/1‑68 (MQ=255) tCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt > 1:1670959/1‑68 (MQ=255) tCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCaa > 1:3228397/1‑59 (MQ=255) tCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCaa > 1:3583699/1‑59 (MQ=255) tCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt > 1:1622790/1‑68 (MQ=255) tCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt > 1:1115516/1‑68 (MQ=255) tCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt > 1:3707544/1‑68 (MQ=255) cTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGtt > 1:3161009/1‑49 (MQ=255) cTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGtt > 1:2698746/1‑49 (MQ=255) cTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGtt < 1:2692516/49‑1 (MQ=255) cTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGtt > 1:1571204/1‑49 (MQ=255) cTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGa > 1:1983260/1‑51 (MQ=255) | GCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCT > W3110S.gb/1999870‑1999997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |