| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,000,742 | C→T | 13.1% | M292I (ATG→ATA) | yedO ← | D‑cysteine desulfhydrase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,000,742 | 0 | C | T | 13.1% | 103.8 / 7.1 | 38 | M292I (ATG→ATA) | yedO | D‑cysteine desulfhydrase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (2/3); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
AATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTC > W3110S.gb/2000679‑2000810 | aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTTATACCGTCAATCAGCCCCGCTATCGc < 1:3211655/69‑1 (MQ=255)aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGc > 1:2470100/1‑69 (MQ=255)aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGc > 1:1861645/1‑69 (MQ=255) aGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt > 1:2852552/1‑66 (MQ=255) aaTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:600554/69‑1 (MQ=255) aaTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:437617/69‑1 (MQ=255) aaTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:300887/69‑1 (MQ=255) aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGta < 1:2279421/69‑1 (MQ=255) aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:296592/68‑1 (MQ=255) cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtata < 1:3110024/69‑1 (MQ=255) cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtata > 1:2767967/1‑69 (MQ=255) cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGa > 1:2315981/1‑68 (MQ=255) aTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:3032896/43‑1 (MQ=255) aTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:1596686/43‑1 (MQ=255) aTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:1128681/43‑1 (MQ=255) tCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:1252645/42‑1 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg > 1:260310/1‑53 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATaca < 1:1580651/59‑1 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa > 1:3434878/1‑67 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa > 1:3440333/1‑67 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa > 1:402427/1‑67 (MQ=255) aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1139182/68‑1 (MQ=255) aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1510414/68‑1 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCt > 1:2382947/1‑68 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATacac < 1:3523175/49‑1 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCa < 1:2908034/60‑1 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1745024/61‑1 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCt > 1:1524361/1‑68 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAAACAGGATCAAGCAg < 1:3091697/61‑1 (MQ=255) ccGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCa < 1:1793047/49‑1 (MQ=255) ccGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc > 1:2225875/1‑69 (MQ=255) ccGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc > 1:223445/1‑69 (MQ=255) aaTCAGCCCCGCCATCGCTTTTCCGGTATTCACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa > 1:122907/1‑69 (MQ=255) aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1976641/45‑1 (MQ=255) aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg > 1:2457359/1‑45 (MQ=255) aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:2726101/45‑1 (MQ=255) aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa > 1:3021567/1‑69 (MQ=255) aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa > 1:2889490/1‑69 (MQ=255) cAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt < 1:1325279/69‑1 (MQ=255) aGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTc < 1:3529932/69‑1 (MQ=255) aGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCa > 1:1381249/1‑64 (MQ=255) aGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt > 1:1717552/1‑68 (MQ=255) cATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc < 1:1877358/69‑1 (MQ=255) cATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc < 1:2215065/69‑1 (MQ=255) | AATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTC > W3110S.gb/2000679‑2000810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |