| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,069,625 | C→T | 10.4% | intergenic (‑167/‑1147) | insH ← / → yoeA | IS5 transposase and trans‑activator/CP4‑44 prophage region; ECK1989:JW5326:b1995; predicted disrupted hemin or colicin receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,069,625 | 0 | C | T | 10.4% | 143.2 / 6.5 | 48 | intergenic (‑167/‑1147) | insH/yoeA | IS5 transposase and trans‑activator/CP4‑44 prophage region; ECK1989:JW5326:b1995; predicted disrupted hemin or colicin receptor |
| Reads supporting (aligned to +/- strand): ref base C (25/18); new base T (3/2); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGAT > W3110S.gb/2069561‑2069691 | tttATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCg > 1:3063494/1‑68 (MQ=255)tttATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCg > 1:3280980/1‑68 (MQ=255) ttattaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCt > 1:3241814/1‑69 (MQ=255) ttattaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCt > 1:2730772/1‑69 (MQ=255) ttattaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCt < 1:1975983/69‑1 (MQ=255) accaGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACAtcatca > 1:2056556/1‑68 (MQ=255) aGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGa > 1:1499847/1‑68 (MQ=255) gTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCAtcttct < 1:2093805/47‑1 (MQ=255) gTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATa > 1:196483/1‑67 (MQ=255) tAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACatcatc < 1:2706222/57‑1 (MQ=255) tAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAg > 1:1518238/1‑69 (MQ=255) aCCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAgg < 1:2813024/68‑1 (MQ=255) aCCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAgg < 1:2773962/68‑1 (MQ=255) ggTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTAt > 1:1757442/1‑69 (MQ=255) gTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTAt > 1:2813623/1‑68 (MQ=255) gTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATaaa > 1:57862/1‑59 (MQ=255) gTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATaaa > 1:2696552/1‑59 (MQ=255) gTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATaaa > 1:3410035/1‑59 (MQ=255) gTAACCACGACCCAGTCGGGGATATACTCCATCTTCTCGCTGACATCATCAAGAATaa > 1:1614191/1‑58 (MQ=255) tAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATaaa < 1:1430570/58‑1 (MQ=255) ccACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATaaa < 1:1476517/55‑1 (MQ=255) cACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTa < 1:2323638/62‑1 (MQ=255) cACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATcac < 1:653583/66‑1 (MQ=255) cACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATcac < 1:3531431/66‑1 (MQ=255) cACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATcac < 1:2092822/66‑1 (MQ=255) cACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATcac < 1:1300394/66‑1 (MQ=255) aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCaa > 1:2110000/1‑69 (MQ=255) aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCaa > 1:3418446/1‑69 (MQ=255) cGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATaaaaa > 1:2547497/1‑45 (MQ=255) ggggATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCag > 1:2076712/1‑60 (MQ=255) ggggATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCag > 1:265352/1‑60 (MQ=255) ggATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGttc < 1:1703753/68‑1 (MQ=255) tatatTCAATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACg > 1:43588/1‑62 (MQ=255) atatTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag < 1:207499/69‑1 (MQ=255) atatTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag < 1:3625286/69‑1 (MQ=255) tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAgg < 1:3465810/39‑1 (MQ=255) tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAgg < 1:3321367/39‑1 (MQ=255) tatTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagc > 1:1553582/1‑69 (MQ=255) tatTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagc > 1:152518/1‑69 (MQ=255) atTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagca > 1:1709064/1‑69 (MQ=255) atTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagca > 1:787876/1‑69 (MQ=255) ttCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGt > 1:2608534/1‑38 (MQ=255) ttCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag > 1:3277834/1‑69 (MQ=255) tCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCacac > 1:1530778/1‑46 (MQ=255) tCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCacac > 1:797081/1‑46 (MQ=255) ccATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag < 1:2108029/64‑1 (MQ=255) ccATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag < 1:2578917/64‑1 (MQ=255) ccATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag < 1:7877/64‑1 (MQ=255) ccATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag < 1:1016664/64‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt < 1:1255548/41‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt < 1:849894/41‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt < 1:468777/41‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt < 1:2792164/41‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt < 1:2014693/41‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt < 1:1832421/41‑1 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGAt > 1:1524639/1‑69 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGAt > 1:151206/1‑69 (MQ=255) ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGAt > 1:937062/1‑69 (MQ=255) | TTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGAT > W3110S.gb/2069561‑2069691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |